食用仙人掌‘米邦塔’的试管快繁

 对食用仙人掌‘米邦塔’的试管快繁研究结果表明: 初代培养基以MS + 6-BA 0.6 mg·L^-1 +NAA 1.0 mg·L^-1 + 2 ,4-D 0.1 mg·L^-1为最佳处理, 接种60 d 后, 外植体上刺座下的潜伏芽萌发率可达67 %。继代增殖培养适宜培养基为MS + 6-BA 0.1 mg·L^-1 + NAA 1.0 mg·L^-1 + 2 ,4-D 0.2 mg·L^-1 或MS + 6-BA 0.1mg·L^-1 + NAA 0.5 mg·L^-1 , 平均每50 d 继代增殖培养1 次, 增殖倍数可达5～6 , 且新生的小子茎生长旺盛。在培养过程中, 虽然部分外植体切口处发生大团绿色至黄绿色小颗粒状愈伤组织, 然而均没有分化不定芽。在生根培养基MS + IAA 0.1 mg·L^-1 或MS + IBA 0.1 mg·L^-1 上, 2～3 cm 高的小子茎培养10 d 左右发根,形成完整植株。当试管苗高达5 cm时移入砂床, 成活率高达99 %, 经约60 d , 试管苗发出第一批掌片。

Rapid Propagation of Cactus'Milpa Alta'in Vitro

Effects of several factors such as medium, cutting materials and sterilization methods for rapid propagation in vitro on cactus ( Opuntia dillenii ) ‘Milpa Alta’were studied. The results indicated that the optimal mediumfor primary culture was MS + 6-BA 0.6 mg·L^-1 + NAA 1.0 mg·L^-1 + 2 ,4-D 011 mg·L^-1 . Taking cactus fresh stem as explant , a shooting rate of 67 %of latent bud under the spine-base was investigated after 60 days of inoculation on this medium. The appropriate mediumfor multiplication and subculture of shooting were MS + 6-BA 0.1 mg·L^-1 +NAA 1.0 mg·L^-1 + 2 ,4-D 0.2 mg·L^-1 or MS + 6-BA 0.1 mg·L^-1 +NAA 0.5 mg·L^-1 . On this medium shoot multiplication occured at a 5 to 6-fold rate after 50 days. The shoots cultured on the rooting medium of MS + IAA 0.1 mg·L^-1 or MS + IBA 0.1 mg·L^-1 produced rootlets after 10 days. The plantlet upwards of 5 cm height was rooted in sand medium with up to 99 % survival and produced new shoots in the field after 60 days.