| 不同番茄品种再生体系的比较 |
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| 引用本文： | 赵明珠,张美萍.不同番茄品种再生体系的比较[J].北方园艺,2011(9):127-129. |
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| 作者姓名： | 赵明珠 张美萍 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林,长春,130118;吉林农业大学生命科学学院,吉林,长春,130118 |
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| 基金项目： | 吉林省教育厅“十一五”科学技术研究资助项目(吉教科合字[2009]第48号) |
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| 摘 要： | 以"大红番茄"、"红牛奶番茄""粉冠888"3种番茄为试材,选取7 d苗龄无菌苗的子叶和胚轴为外植体,对外植体基因型、分化培养基及生根培养基进行筛选,建立番茄再生体系,以期为进一步进行人参大片段DNA转化番茄的研究奠定基础。结果表明:"粉冠888"可为遗传转化提供大量稳定的外植体来源,适宜的分化培养基为MS+6-BA 1.0~2.0 mg/L+IAA 0.2 mg/L,生根培养基为MS+IAA 0.5 mg/L。
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| 关 键 词： | 番茄 再生体系 培养基 |
Comparison in Plant Regeneration of Different Varieties of Tomato |
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| ZHAO Ming-zhu,ZHANG Mei-ping.Comparison in Plant Regeneration of Different Varieties of Tomato[J].Northern Horticulture,2011(9):127-129. |
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| Authors: | ZHAO Ming-zhu ZHANG Mei-ping |
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| Institution: | ZHAO Ming-zhu,ZHANG Mei-ping(College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Using three kinds of tomatoes as material,7 old days in vitro seedling cotyledon and hypocotyls were used as explant to filter genotype,differential medium and root medium in order to establish tomato regeneration system.The next step was using ginseng large DNA fragment transform tomato.The results showed that ’Fenguan 888’could provide lots of and stable explant,the suitable differentiation medium was MS+6-BA 1.0～2.0 mg/L+IAA 0.2 mg/L,and the suitable rooting medium was MS+IAA 0.5 mg/L. |
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| Keywords: | tomato regeneration system medium |
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