| 石海椒愈伤组织诱导与增殖研究 |
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| 引用本文： | 古玉,许强,李龙,GU Yu,XU Qiang,LI Long. 石海椒愈伤组织诱导与增殖研究[J]. 北方园艺, 2011, 0(9): 130-134 |
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| 作者姓名： | 古玉 许强 李龙 GU Yu XU Qiang LI Long |
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| 作者单位： | 四川农业大学生命科学与理学院,四川,雅安,625014 |
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| 基金项目： | 四川农业大学教学质量推进计划资助项目 |
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| 摘 要： | 对石海椒幼嫩叶片和茎段进行了愈伤组织的诱导增殖研究,以期探寻其诱导和增殖的最佳条件。结果表明:石海椒的叶片和茎段都能诱导出愈伤组织,但最佳外植体为叶片,叶片和茎段在0.1%升汞中适合消毒时间为6 min和10 min。叶片最适诱导培养基为B5+0.6 mg/L6-BA+2.0 mg/L NAA,增殖培养基为B5+0.6 mg/L 6-BA+1.25 mg/L NAA。基本培养基和生长调节剂对愈伤诱导的影响是NAA>6-BA>基本培养基。
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| 关 键 词： | 石海椒 愈伤组织 诱导条件 增殖条件 |
Study on Callus Induction and Proliferatation of Reinwardtia trigyna(Roxb.) Planch |
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| GU Yu,XU Qiang,LI Long. Study on Callus Induction and Proliferatation of Reinwardtia trigyna(Roxb.) Planch[J]. Northern Horticulture, 2011, 0(9): 130-134 |
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| Authors: | GU Yu XU Qiang LI Long |
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| Affiliation: | GU Yu,XU Qiang,LI Long(College of Biology and Science,Sichuan Agricultural University,Yaan,Sichuan 625014) |
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| Abstract: | The leaves and stems of Reinwardtia trigyna(Roxb.) Planch were used as explants to study the callus induction and proliferation,in order to explore the optimum conditions of in duction and proliferation.The results indicated that both leaves and stems could induce callus,but the best explant was leaf.The suitable sterilization time for leaves and stems with 0.1% mercury bichloride were 6 min and 10 min.The optimal induction medium for leaf were B5+0.6 mg/L 6-BA+2.0 mg/L NAA,and proliferation medium were B5+... |
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| Keywords: | Reinwardtia trigyna(Roxb.) Planch callus induction condition proliferation condition |
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