| 番茄组织培养中无菌苗培养条件的优化 |
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| 引用本文： | 吴志刚,宋 明,王志敏,牛 义. 番茄组织培养中无菌苗培养条件的优化[J]. 中国农学通报, 2006, 22(4): 335-335 |
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| 作者姓名： | 吴志刚 宋 明 王志敏 牛 义 |
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| 作者单位： | 西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆,400716 |
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| 摘 要： | 对番茄组织培养中种子的消毒方法和无菌苗培养条件进行了优化。经过对五种消毒方法的消毒效果比较，以10%的NaClO溶液+土温1滴30min和10%NaClO溶液20 min + 0.1%HgCl2 3 min消毒效果最佳，对种子发芽抑制作用较小。同时，通过对无菌苗生理指标的测定，添加1%蔗糖的1/2MS培养基有利于壮苗的形成。
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| 关 键 词： | 帐幕熏蒸 帐幕熏蒸 研 究 |
| 收稿时间： | 2005-12-05 |
| 修稿时间： | 2005-12-052005-12-08 |
Condition Optimization for Culturing Sterile Tomato Plants In Tissue Culture of Tomato ( Lycopersicon esculentum Miller ) |
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| Wu Zhigang,Song Ming,Wang Zhimin,Niu Yi. Condition Optimization for Culturing Sterile Tomato Plants In Tissue Culture of Tomato ( Lycopersicon esculentum Miller )[J]. Chinese Agricultural Science Bulletin, 2006, 22(4): 335-335 |
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| Authors: | Wu Zhigang Song Ming Wang Zhimin Niu Yi |
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| Affiliation: | Key Lab in Vegetable Research of Chongqing, College of Horticulture and Landscape, Southwest University, Chongqing 400716 |
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| Abstract: | In this paper, the method of seed sterilization and culture condition of sterile tomato plants are optimized. The better sterilization method with little restrain to germination is 10%NaClO Tween one drop 30minutes or 10%NaClO 20 min 0.1%HgCl2 3 min; And the sterile plants in the1/2 MS medium with 1% sucrose are more healthy by comparing their physiological index. |
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| Keywords: | Tomato Tissue culture Sterile Plant |
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