Identification and Characterization of Diploid and Tetraploid in <Emphasis Type="Italic">Platycodon grandiflorum</Emphasis> |
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| Authors: | Jeoung-Hwa Shin Yun Gyong Ahn Ju-Hee Jung Sun-Hee Woo Hag-Hyun Kim Shela Gorinstein Hee-Ock Boo |
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| Institution: | 1.Seoul Center,Korea Basic Science Institute,Seoul,Republic of Korea;2.Western Seoul Center,Korea Basic Science Institute,Seoul,South Korea;3.Department of Crop Science,Chungbuk National University,Cheongju,South Korea;4.Faculty of Food Nutrition and Cookery,Woosong Information College,Daejeon,South Korea;5.Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School,Jerusalem,Israel;6.PHYTO M&F Co. Ltd., BI Center, GIST,Gwangju,South Korea |
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| Abstract: | Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution – time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars. |
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