驴生长激素基因cDNA的克隆及生物信息学分析

目的]研究驴GH基因的特性与功能。方法]以驴肝组织为材料,设计一对特异性引物,采用RT-PCR技术克隆驴GH基因cDNA序列。结果]测序得到706bp的驴GH基因cDNA序列,此序列包含完整的GH基因的阅读框,编码216个氨基酸,驴GH基因编码的蛋白有7个的疏水区域,存在7个跨膜区和信号肽,二级结构主要以α-螺旋和不规则卷曲为主,三级结构包含第27～215位氨基酸残基。结论]驴GH基因在进化上非常保守,基于CDS序列构建的系统进化树与比较形态学和比较生理学结果一致。

Cloning and Bioinformatics Analysis of Donkey Growth Hormone Gene cDNA

Objective] The paper aimed to study the donkey GH gene features and functions.Method] A pair of specific PCR primers was designed for cloning the coding sequence of the donkey GH gene from liver tissue.Result] 706 bp fragment was got by RT-PCR amplification.The sequence included a complete open reading frame and encoded 216 amino acids.The protein encoded by donkey GH gene had seven hydrophobic region,seven transmembrane regions and a signal peptide;it’s secondary structure had α-helix and irregular curl and three-dimensional solution structure composed of 27-215 amino acids.Conclusion] GH gene of donkey was very conservative in evolution.The phylogenetic tree constructed basing on CDS sequence is consistent with the results of comparative morphology and comparative physiology.