A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis |
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| Authors: | Marí a Sol Arias, Jos Luis Su rez, G.V. Hillyer, Iv n Francisco, Enrique Calvo, Rita S nchez-Andrade, Pablo Dí az, Rub n Francisco, Pablo Dí ez-Ba os, Patrocinio Morrondo,Adolfo Paz-Silva |
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| Affiliation: | aAnimal Pathology Department, Epidemiology, Zoonoses and Parasitic Diseases, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain;bLaboratory of Parasite Immunology and Pathology, School of Medicine, Medical Sciences Campus, University of Puerto Rico, San Juan, PR 00936-5067, USA;cAnimal Health Division, Schering-Plough S.A. C/Cantabria, No. 2 Planta 3ª Edificio Amura, Alcobendas, 28108 Madrid, Spain |
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| Abstract: | The therapeutic efficacy of albendazole and netobimin in ruminants with naturally occurring fascioliasis was investigated using a recombinant-based ELISA. The variation in the IgG response against a 2.9-kDa recombinant protein (FhrAPS), termed efficacy index (EI) 1, and the egg-output changes, termed EI 2, were used to evaluate drug efficacy.The values of EI 1 ranged between 0% and 50% in sheep, and between 0% and 30% in cattle after treatment with albendazole and netobimin. Similar EI 2 values were observed in sheep receiving albendazole or netobimin, but the highest values were found in cattle treated with netobimin. The significant reduction in the IgG response to FhrAPS found in this study shows promise in terms of developing alternative methods for evaluating the efficacy of chemotherapy against Fasciola hepatica in grazing ruminants. |
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| Keywords: | Fascioliasis Ruminants Chemoprophylaxis Efficacy index Recombinant protein FhrAPS |
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