太湖鸡资源群体遗传变异的检测

应用微卫星标记对江苏省无锡市祖代鸡场有限公司太湖鸡的保种群体进行了遗传变异的分析,计算了群体的杂和度(H)和多态信息含量(PIC)。研究结果表明:30个微卫星标记在太湖鸡保种群体的杂和度为0.504～0.858之间,平均杂和度为0.688,群体的遗传多样性丰富;其中24个微卫星标记在太湖鸡中多态信息含量为高度多态,可作为有效遗传标记用于太湖鸡保种群的遗传分析,而且各座位杂和度的高低与PIC值的大小体现了较高的一致性。本研究结果为太湖鸡开发利用研究提供了基础,也为太湖鸡的合理保护提供了科学根据。

Analysis on Molecular Genetic Diversity of Preserved Population of Taihu Chicken

The genetic diversity of heterozygosity and polymorphic information content of preserved population of Taihu chicken in breeding chicken field of Wuxi city of Jiangsu province was analyzed by 30 microsatellite loci with high polymorphism. The results showed that the heterozygosity range of the 30 microsatellite loci was from 0.504 to 0.858,the mean heterozygosity was 0.688. It reflected that Taihu chicken had rich diversity. 24 of 30 microsatellite loci were high polymorphic,so they were effective markers for genetic analysis of preserved population of Taihu chicken. The order of the heterozygodity was almost consistent with that of the polymorphism information content(PIC). Those results could provide basic molecular data for the research and scientific basis for the conservation and utilization of Taihu chicken.