| 颗粒细胞单层对黄牛孤雌胚胎体外发育的影响 |
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| 引用本文： | 彭辉,卿素珠,李莉,肖红卫,乔宪凤,郑新民. 颗粒细胞单层对黄牛孤雌胚胎体外发育的影响[J]. 湖北农业科学, 2009, 48(4) |
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| 作者姓名： | 彭辉 卿素珠 李莉 肖红卫 乔宪凤 郑新民 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西,杨凌,712100;湖北省农业科学院畜牧兽医研究所/湖北省动物胚胎工程及分子育种重点实验室,武汉,430064 |
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| 基金项目： | 湖北省科技攻关项目，湖北省重点实验室开放课题 |
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| 摘 要： | 为优化颗粒细胞单层共培养体系,研究了不同类型、不同种属的颗粒细胞单层及不同时间更换培养单层对黄牛孤雌胚胎体外发育的影响.结果表明.就卵裂率、囊胚率和囊胚孵出率而言.壁颗粒细胞单层组与丘颗粒细胞单层组之间无显著差异(P>0.05);来自黄牛、猪和小鼠的颗粒细胞单层在支持黄牛孤雌胚胎体外发育方面无显著差异(P>0.05):就囊胚率而言,共培养的第3天和第6天两次更换单层分别与共培养的第4天更换单层和始终不更换单层的对照组之间无显著差异(P>0.05),但第4天更换单层与对照组之间有显著差异(P<0.05).所以.不同类型和不同种属的颗粒细胞单层都能很好地支持黄牛孤雌胚胎的体外发育.在胚胎的体外发育过程中更换培养单层可以明显提高胚胎的囊胚率和囊胚孵出率,并且在共培养的第4天更换培养单层可以得到较高的囊胚率.
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| 关 键 词： | 黄牛 孤雌胚胎 颗粒细胞单层 体外培养 |
Effect of Cumulus Cell Monolayers on In Vitro Development of Cattle Parthenogenetic Embryos |
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| PENG Hui,QING Su-zhu,LI Li,XIAO Hong-wei,QIAO Xian-feng,ZHENG Xin-min. Effect of Cumulus Cell Monolayers on In Vitro Development of Cattle Parthenogenetic Embryos[J]. Hubei Agricultural Sciences, 2009, 48(4) |
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| Authors: | PENG Hui QING Su-zhu LI Li XIAO Hong-wei QIAO Xian-feng ZHENG Xin-min |
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| Affiliation: | 1. Medical College of Animal;Northwest Sci-Tech University of Agriculture and Forestry;Yangling 712100;Shaanxi;China;2.Institute of Animal Husbandry and Vetervinary Sciences;Hubei Academy of Agricultural Sciences / Hubei Key Lab of Animal Embryo and Molecular Breeding;Wuhan 430064;China |
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| Abstract: | To optimize co-culture system of cumulus cell monolayers, effects of different type, species and renew time of cumulus cell monolayers on in vitro development of cattle parthenogenetic embryos were observed. The results indicated that there were no notable difference between granulosa cell monolayers group and cumulus cell monolayers group for the cleavage, blastocyst and hatched rate (P>0.05). The developmental ability of parthenogenetic embryos co-cultured with cattle cumulus cell monolayers was no differ... |
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| Keywords: | cattle parthenogenetic embryos cumulus cell monolayers in vitro culture |
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