5株猪流行性腹泻病毒流行株的遗传变异分析

为了解当前猪流行性腹泻流行情况,对4个地区疑似PEDV S1、ORF3基因进行RT-PCR扩增,并对5株PEDV流行株进行核苷酸同源性及遗传进化分析。S1基因核苷酸同源性比较与进化分析显示,5株PEDV流行株与意大利Italy/69979-25株、美国OH851株、我国主要变异株W-pintung-52株、AH2012、JXGZ2013等位于同一进化分支,其核苷酸同源性达98%以上。ORF3基因核苷酸同源性比较与进化分析发现,5株PEDV流行株均属于野毒株,与Tottori/JPN/2014变异株亲缘关系较近,其核苷酸同源性高达98.8%～100%。提示当前猪流行性腹泻病毒仍以变异株为主。

Genetic variation analysis of epidemic strains of porcine epidemic diarrhea virus

To understand the current epidemic situation of porcine epidemic diarrhea,the genetic variation of S1 and ORF3 genes for the suspected PED epidemic strains in 4 areas were analyzed by the method of RT-PCR.The S1 gene phylogenetic and nucleotide homology analysis showed that the five strains of PEDV have closed genetic relationship with Italy/69979-25、OH851strains of American、TW-pintung-52、AH2012 and JXGZ2013 strains of chinese variant,whose nucleotide homology was higher than 98%.Genetic evolution and nucleotide homology analysis of ORF3 gene showed that these strains were all wild strains and were closely related to the Tottori/JPN/2014 variant.the nucleotide homology was 98.8%-100%;the result suggested that the porcine epidemic diarrhea virus is mainly variation.