|
|||||
|
|
| 不同花生品种胚小叶芽诱导的研究 | |
| 引用本文： | 任艳,李磊,崔潇,石延茂,王辉,李双铃,袁美.不同花生品种胚小叶芽诱导的研究[J].山东农业科学,2012,44(7):13-15. |
| 作者姓名： | 任艳 李磊 崔潇 石延茂 王辉 李双铃 袁美 |
| 作者单位： | 1. 中国海洋大学,山东青岛266100;山东省花生研究所,山东青岛266100 2. 青岛市农业科学研究院,山东青岛,266100 3. 山东省花生研究所,山东青岛,266100 |
| 基金项目： | 科技部"863"计划项目 |
| 摘 要： | 比较了4个花生品种的胚小叶在不同浓度NAA和TDZ诱导培养基中的不定芽诱导率;并观察了用0.1%升汞和10%次氯酸钠不同处理的灭菌效果及其对种子萌发的影响。结果表明:花生种子用75%酒精处理0.5～1 min后用10%次氯酸钠处理15 min灭菌效果理想,萌发率高;诱导培养基中以添加1 mg/LNAA和0.02 mg/L TDZ最有利于不定芽诱导,4个花生品种诱导率都较高,达到80%以上,其中花育20的芽诱导率最高,为89.2%。 |
| 关 键 词： | 花生品种 组织培养 种子萌发 芽诱导 |
Study on Shoot Induction from Leaflets of Different Peanut Cultivars (Arachis hypogaea L.) |
|
| REN Yan , LI Lei , CUI Xiao , SHI Yan-mao , WANG Hui , LI Shuang-ling , YUAN Mei.Study on Shoot Induction from Leaflets of Different Peanut Cultivars (Arachis hypogaea L.)[J].Shandong Agricultural Sciences,2012,44(7):13-15. | |
| Authors: | REN Yan LI Lei CUI Xiao SHI Yan-mao WANG Hui LI Shuang-ling YUAN Mei |
| Institution: | 1.Ocean University of China,Qingdao 266100,China;2.Shangdong Peanut Research Institute,Qingdao 266100,China; 3.Qingdao Academy of Agricultural Sciences,Qingdao 266100,China) |
| Abstract: | The shoot induction rate of lobules embryos from 4 peanut cultivars were compared in the media added different concentrations of NAA and TDZ.The sterilizing effect of 0.1% HgCl2 and 10% NaClO and their influences to seed germination were also observed.The results indicated that the sterilizing effect was ideal and the germination rate was higher when the peanut seeds were treated for 0.5～1.0 minutes with alcohol and 15 minutes with 10% NaClO.The media added 1 mg/L NAA and 0.02 mg/LTDZ were the most favorable to in vitro shoot induction with the induction rate over 80%.In which,the shoot induction rate of Huayu 20 was the highest as 89.2%. |
| Keywords: | Peanut cultivar Tissue culture Seed germination Shoot induction |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |