| 苹果香气SPME-GC/MS萃取条件优化 |
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| 引用本文： | 王传增,张艳敏,徐玉亭,董飞,宋杨,刘美艳,刘金,陈学森. 苹果香气SPME-GC/MS萃取条件优化[J]. 山东农业科学, 2012, 44(7): 116-120 |
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| 作者姓名： | 王传增 张艳敏 徐玉亭 董飞 宋杨 刘美艳 刘金 陈学森 |
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| 作者单位： | 山东农业大学/作物生物学国家重点实验室,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金项目，国家重点基础研究发展计划项目 |
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| 摘 要： | 以富士为试材,利用固相微萃取(SPME)气相色谱-质谱(GC/MS)联用技术分析苹果香气组分,探讨了不同萃取头、萃取温度、萃取时间和解吸时间等条件对分析结果的影响。结果表明:苹果的SPME-GC/MS最优萃取方法为,用DVB/CAR/PDMS(50/30μm)萃取头在50℃下萃取35 min后,高温解吸2 min,能有效吸附苹果果实香气;重现性分析显示,主要香气成分的变异系数均小于10%,且大部分在5%左右,重现性良好。
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| 关 键 词： | 苹果 香气成分 SPME-GC/MS 萃取条件优化 |
Optimization of Extracting Conditions for Apple Aroma by SPME-GC/MS |
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| WANG Chuan-zeng , ZHANG Yan-min , XU Yu-ting , DONG Fei , SONG Yang , LIU Mei-yan , LIU Jin , CHEN Xue-sen. Optimization of Extracting Conditions for Apple Aroma by SPME-GC/MS[J]. Shandong Agricultural Sciences, 2012, 44(7): 116-120 |
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| Authors: | WANG Chuan-zeng ZHANG Yan-min XU Yu-ting DONG Fei SONG Yang LIU Mei-yan LIU Jin CHEN Xue-sen |
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| Affiliation: | (Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,China) |
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| Abstract: | The aroma components of ’Fuji’ apple were analyzed using solid phase microextraction(SPME) and gas chromatography-mass spectrometry(GC/MS).The effects of fibers,extraction temperature,extraction time,desorption time on the results were discussed.The results were as follows.The optimal conditions for the aroma extraction from apple fruits were using DVB/CAR/PDMS(50/30 μm) fiber to extract for 35 minutes under 50℃ and then desorbed for 2 minutes at high temperature,by which,the aroma could be absorbed effectively.The reproducibility analysis showed that the variation coefficients of main aroma components were less than 10% and most about 5%,which showed good reproducibility. |
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| Keywords: | Apples Aroma components SPME-GC/MS Optimization of extraction conditions |
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