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| 向日葵抗坏血酸过氧化物酶的电子克隆和生物信息学分析 | |
| 引用本文： | 孟鹏,李根英,陈国强,王振东.向日葵抗坏血酸过氧化物酶的电子克隆和生物信息学分析[J].山东农业科学,2012,44(6):1-6. |
| 作者姓名： | 孟鹏 李根英 陈国强 王振东 |
| 作者单位： | 1. 沈阳农业大学生物科学技术学院,辽宁沈阳,110161 2. 山东省农业科学院作物研究所,山东济南,250100 3. 淮海工学院/江苏省海洋生物技术重点实验室,江苏连云港,222005 |
| 基金项目： | 农业部，转基因生物新品种培育重大专项，国家自然科学基金 |
| 摘 要： | 利用电子克隆技术得到长753 bp的向日葵抗坏血酸过氧化物酶(HaAPX)cDNA。通过生物信息学分析发现,HaAPX在核酸水平和蛋白质水平上都与常见物种的APX非常相似,具有极其保守的酶活作用域Peroxidase-1(-divtlsggHtl-)和Peroxidase-2(-aplmlRlawHsa-),且配体结合位点和金属结合位点完全保守。HaAPX与甘薯(Ipomoea batatas)APX亲缘关系很近,其编码蛋白由250个氨基酸组成,分子量为2.7 ku,无信号肽和跨膜区,二级结构由37.6%α-螺旋、13.2%β-折叠、9.2%β-转角和40%无规则卷曲组成,三维构象与豌豆APX的非常相似。 |
| 关 键 词： | 向日葵 抗坏血酸过氧化物酶 电子克隆 生物信息学 |
In Silicon Cloning and Bioinformatic Analysis of Ascorbate Peroxidase Gene in Helianthus annuus |
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| MENG Peng , LI Gen-ying , CHEN Guo-Qiang , WANG Zhen-dong.In Silicon Cloning and Bioinformatic Analysis of Ascorbate Peroxidase Gene in Helianthus annuus[J].Shandong Agricultural Sciences,2012,44(6):1-6. | |
| Authors: | MENG Peng LI Gen-ying CHEN Guo-Qiang WANG Zhen-dong |
| Institution: | 1 (1.College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China; 2.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Huaihai Institution of Technology/Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang 222005,China) |
| Abstract: | Using in silicon cloning,the cDNA of ascorbate peroxidase（APX） was cloned from Helianthus annuus with the full length as 753 bp.The bioinformatic analysis showed that the HaAPX shared a high similarity with that of other species in DNA and protein level,possessing the domains of Peroxidase1（-divtlsggHtl-） and Peroxidase2（-aplmlRlawHsa-）,as well as the ligand-binding sites and metal-binding sites were extremely conserved.HaAPX was closer to APX of Ipomoea batatas in the phylogenetic tree.HaAPX encoded a protein of 250 amino acids with the molecular weight as 2.7 ku.The signal peptide and trans-membrane region were not found in the protein sequence.The protein contains 37.6% of α-helix,13.2% of β-sheet,9.2% of β-turns and 40% of random coil.The 3-D conformation of HaAPX protein was in accordance with APX of Pisum sativum. |
| Keywords: | Helianthus annuus Ascorbate peroxidase In silicon cloning Bioinformatics |
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