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| 犬冠状病毒的分离鉴定及其S基因的克隆 | |
| 引用本文： | 廖列如,张林,孔庆波,刘国权,孙彩虹,张淼涛. 犬冠状病毒的分离鉴定及其S基因的克隆[J]. 西北农业学报, 2008, 17(2): 33-37,42 |
| 作者姓名： | 廖列如 张林 孔庆波 刘国权 孙彩虹 张淼涛 |
| 作者单位： | 1. 西北农林科技大学动物医学院,陕西杨凌,712100 2. 西北农林科技大学动物医学院,陕西杨凌,712100;公安部警犬技术学校,辽宁沈阳,110034 3. 公安部警犬技术学校,辽宁沈阳,110034 4. 西安市动物疫病预防控制中心,陕西西安,710061 |
| 基金项目： | 公安部科技局资助项目(编号:20019829502) |
| 摘 要： | 从疑似犬冠状病毒(CCV)症状病犬粪便中分离出一株病毒,合成通用引物(2Bp,4Bm)和CCV特异性引物(CCVF2,CCVR2)进行RT-PCR反应及限制性内切酶BstXⅠ酶切鉴定,并将纯化的PCR产物成功地克隆到pGEM-T-easy载体中。RT-PCR反应结果,BstXⅠ酶切鉴定结果以及重组质粒PCR鉴定结果均得到预计扩增片段。重组质粒测序结果与GenBank的CCV序列进行比对,与之相符,表明本次实验分离到的病毒为CCV。 |
| 关 键 词： | 犬冠状病毒 分离 鉴定 S基因 克隆 |
| 文章编号： | 1004-1389(2008)02-0033-05 |
| 收稿时间： | 2007-07-05 |
| 修稿时间： | 2007-07-05 |
Isolation and Identification of Canine Coronavirus and Cloning of Partial S Gene |
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| LIAO Lie-ru,ZHANG Lin,KONG Qing-bo,LIU Guo-quan,SUN Cai-hong and ZHANG Miao-tao. Isolation and Identification of Canine Coronavirus and Cloning of Partial S Gene[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(2): 33-37,42 | |
| Authors: | LIAO Lie-ru ZHANG Lin KONG Qing-bo LIU Guo-quan SUN Cai-hong ZHANG Miao-tao |
| Affiliation: | College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China;Police Dog Technical Training School of Public Security Ministry, Shenyang 110034, China;Police Dog Technical Training School of Public Security Ministry, Shenyang 110034, China;Xi''an Animal Disease Prevention and Control Center, Xi''an 710061, China;College of Veterinary Medicine, Northwest A & F University, Yangling Shaanxi 712100, China |
| Abstract: | A strain of virus was isolated from the fecal samples of a dog with clinical CCV illness.RT PCR Identification by use of 2 pairs of common primers 2 Bp and 4 Bm and specific primers CCVF2 and CCVR2 as well as BstX degestion was carried out.PCR products were purified and cloned into the pGEM Teasy vector.The result of RT PCR,Bst X digestion and recombinant plasmid amplified all gained the expect amplified segment.The result of sequencing shows that the PCR product is completely ident ical with that of the sequence searched in gene bank and indicated that the isolated virus is CCV. |
| Keywords: | CCV Isolation Identification S gene Clone |
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