| 纤维素酶辅助提取银杏叶总黄酮的工艺条件 |
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| 引用本文： | 丁兴红,孙杰,喻治霞,罗丽琴,高燕,吕乙铖. 纤维素酶辅助提取银杏叶总黄酮的工艺条件[J]. 林业科技开发, 2009, 23(4): 66-68 |
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| 作者姓名： | 丁兴红 孙杰 喻治霞 罗丽琴 高燕 吕乙铖 |
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| 作者单位： | 浙江中医药大学,杭州,310053 |
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| 基金项目： | 浙江省教育厅科学研究项目，浙江省新苗人才计划项目 |
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| 摘 要： | 研究了纤维素酶辅助提取银杏叶总黄酮工艺条件.对银杏叶粒径、纤维素酶用量、酶解时间进行单因素考察,根据单因素考察结果设计正交试验,以总黄酮得率为指标确定适宜的正交试验参数:银杏叶细粉100目、纤维素酶用量30 FPIU/g银杏叶、酶解时间50 min,该工艺条件下银杏叶总黄酮提取得率为2.51%,与未添加纤维素酶相比,总黄酮得率增加了54.93%.
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| 关 键 词： | 银杏叶 总黄酮 纤维素酶 提取 |
Study on the Cellulase-assistant Extraction of Total Flavonoids from Leaves of Ginkgo biloba |
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| DING Xing-hong,SUN Jie,YU Zhi-xia,LUO Li-qin,GAO Yan,L Yi-cheng. Study on the Cellulase-assistant Extraction of Total Flavonoids from Leaves of Ginkgo biloba[J]. China Forestry Science and Technology, 2009, 23(4): 66-68 |
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| Authors: | DING Xing-hong SUN Jie YU Zhi-xia LUO Li-qin GAO Yan L Yi-cheng |
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| Affiliation: | DING Xing-hong,SUN Jie,YU Zhi-xia,LUO Li-qin,GAO Yan,L(U) Yi-cheng |
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| Abstract: | In order to study the factors affected the yield of total flavonoids extracted from leaves of Ginkgo biloba by using cellulase-assistant extraction(EAE) technology,the single factor experiments were conducted,in which the effect of particle size,extraction time and cellulase amount on the extraction was studied respectively.Based on the experiments above,the particle size,extraction time and cellulase amount used were selected as the factors for the orthogonal experiments to find out the optimum operating c... |
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| Keywords: | Leaves of Ginkgo biloba Total flavonoids Cellulase Extraction |
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