| PCR-RFLP法快速测定种猪氟烷基因型及其使用优点 |
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| 引用本文： | 祁永旺,王昕陟,侯华,张翠,王金旭.PCR-RFLP法快速测定种猪氟烷基因型及其使用优点[J].饲料博览,2011(2). |
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| 作者姓名： | 祁永旺 王昕陟 侯华 张翠 王金旭 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院;沈阳市畜牧兽医研究所; |
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| 基金项目： | 国家科技部十一五科技支撑项目(2007BAD71B011-03) |
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| 摘 要： | 从猪毛中提取DNA,用特异性引物进行PCR-RFLP扩增法对沈阳市畜牧兽医研究所种猪场种猪进行氟烷基因检测。结果表明,在测定的长白、大约克、杜洛克种猪中,发现长白猪HaLn基因的基因频率较高,为7.5%、大约克为2.1%、杜洛克为2.0%。所有种猪不含易引起猪应激综合征的氟烷基因隐形纯合子(nn)。用猪毛进行PCR扩增不仅成本低、速度快,且准确性高、取样方便,可大批量地处理样品。从仔猪开始至成年种猪,均可用毛样进行基因诊断,从而有效防止氟烷基因隐形纯合子(nn)的出现,加快种猪的改良步伐。
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| 关 键 词： | 氟烷基因 PCR-RFLP扩增法 猪应激综合征 |
Determination of Halothane Genotype Pigs by PCR-RFLP Rapidly and its Using Advantages |
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| QI Yongwang,WANG Xinzhi,HOU Hua,ZHANG Cui,WANG Jinxu.Determination of Halothane Genotype Pigs by PCR-RFLP Rapidly and its Using Advantages[J].Feed Review,2011(2). |
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| Authors: | QI Yongwang WANG Xinzhi HOU Hua ZHANG Cui WANG Jinxu |
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| Institution: | QI Yongwang1,WANG Xinzhi1*,HOU Hua1,ZHANG Cui1,WANG Jinxu2 (1. College of the Animal Husbandry and Veterinary,Shenyang Agricultural University,Shenyang 110161,China,2. Shenyang Institute of Animal Husbandry and Veterinary,Shenyang 110034,China) |
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| Abstract: | Extracted from the pig using specific primers for PCR-RFLP amplification of Shenyang Institute of Animal breeding farms. The result showed that the gene landrace Haln gene frequencies was higher 7.5%, Yorkshire 2.1%, Duroc 2.0% in the determination of the landrace, Yorkshire and Duroc. There was no homozygous halothane gene(nn) which lead porcine stress syndrome. There was not only low cost, fast and high accuracy, convenience sampling, sample handling large quantities at one time by PCR amplified with the ... |
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| Keywords: | weaning piglets digestive physiology nutrition |
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