达里诺尔湖和岗更湖瓦氏雅罗鱼同工酶的研究

瓦氏雅罗鱼是一种耐盐碱性很强的鱼类，本文采用聚丙烯酰胺凝胶电泳法(PAGE)比较研究了淡水水域（岗更湖）和盐碱水水域（达里诺尔湖）中瓦氏雅罗鱼的同工酶，旨在明确瓦氏雅罗鱼在盐碱水域和淡水水域中同工酶的不同，为瓦氏雅罗鱼的耐盐碱机理做基础研究，同时也为瓦氏雅罗鱼在内陆水域的引种与放流提供科学的指导依据。通过比较研究达里诺尔湖和岗根湖的瓦氏雅罗鱼(Leuciscus waleckii)骨骼肌、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)的差异，得出结果：两湖中瓦氏雅罗鱼的同工酶表达具有差异性，其中EST图谱的差异最大，LDH、POD的图谱也存在差异性，而MDH图谱差异不显著。试验表明，两湖中瓦氏雅罗鱼的过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)同工酶存在差异性，而苹果酸脱氢酶(MDH)的差异不显著。

Study of Isoenzymes of Leuciscus waleckii in Dalinor-Lake and ganggeng-lake

Leuciscus waleckii is a fish that can undertake salinity strongly, in this paper comparatively studied Isoenzymes of Leuciscus waleckii live in salt water and fresh waters with polyacrylamidegel electrophoresis (PAGE), to find difference of Isoenzymes of Leuciscus waleckii live in salt water and fresh water. By studying ,to provide basic research for mechanism of salinity and give scientific guidance for introduced in inland waters and release of Leuciscus waleckii. Through a comparative study peroxidase (POD), esterase (EST), lactate dehydrogenase (LDH)and malic acid de-hydrogenase (MDH) in muscle, liver and heart tissue of Leuciscus waleckii, the results obtained: Leuciscus waleckii with differences in expression of the isozymes in the two lakes, and EST mapping has the biggest differences between them, LDH and POD are also have differences in the pattern, but MDH pattern no significant difference. Experiments show that peroxidase (POD), esterase (EST)and lactate dehydrogenase (LDH)of Leuciscus waleckii have differences in two lakes, whereas the difference of malic acid de - hydrogenase (MDH) was not significant.