| 复合诱变选育高产纤维素酶绿色木霉菌株 |
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| 引用本文： | 韩立亚,冯培勇,张玉香,张丽. 复合诱变选育高产纤维素酶绿色木霉菌株[J]. 安徽农业科学, 2009, 37(9): 3869-3870 |
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| 作者姓名： | 韩立亚 冯培勇 张玉香 张丽 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025;鲁东大学生命科学学院,山东烟台,264025;鲁东大学生命科学学院,山东烟台,264025;鲁东大学生命科学学院,山东烟台,264025 |
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| 摘 要： | [目的]选育高纤维素酶活的绿色木霉菌株。[方法]以一株绿色木霉F1为出发菌株,以每一轮诱变处理筛选到的酶活最高的突变株作为下一轮诱变的出发菌株。经过紫外线、亚硝基胍（NTG）、硫酸二乙酯（DES）诱变处理。计录菌落数,计算致死率并测定CMCase的酶活。[结果]紫外线处理200 s时,孢子的致死率接近100%。U1突变株的相对酶活最高,为出发菌株F1的110.4%。NTG处理50 min时,孢子的致死率接近100%,N4诱变菌株的相对酶活最高,为出发菌株F1的128.9%。DES处理50 min时的诱变致死率接近100%,D8菌株的配对酶活最高,相对酶活为出发菌株F1的140.6%。[结论]得到了一株产酶量高且性状稳定的高产绿色木霉菌株D8,其CMCase相对酶活较出发菌株F1提高了40.6%。
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| 关 键 词： | 绿色木霉 纤维素酶 复合诱变 |
Selection and Breeding of Trichoderma viride Strains with High Productivity on Cellulase by Compound Mutation |
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| HAN Li-ya et al. Selection and Breeding of Trichoderma viride Strains with High Productivity on Cellulase by Compound Mutation[J]. Journal of Anhui Agricultural Sciences, 2009, 37(9): 3869-3870 |
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| Authors: | HAN Li-ya et al |
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| Affiliation: | HAN Li-ya et al (Life Science College of Ludong University,Yantai,Sh,ong 264025) |
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| Abstract: | [Objective] The aim was to screen Trichoderma viride strain with high cellulase activity.[Method] With a T.viride strain as the original strain,the screened mutant with the highest enzyme activity in each mutation treatment was used as the original strain in the next mutation.After mutation by ultraviolet(UV),nitrosoguanidine(NTG),diethyl sulfate(DES),the number of colony was recorded,the lethality of the colony was calculated and its CMCase activity was determined.[Result]When the spores were treated by UV... |
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| Keywords: | Trichoderma viride Cellulase Compound mutation |
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