Feline Immunodeficiency Virus and Puma Lentivirus in Florida panthers (Puma concolor coryi): Epidemiology and Diagnostic Issues |
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| Authors: | D. L. Miller S. K. Taylor D. S. Rotstein M. B. Pough M. C. Barr C. A. Baldwin M. Cunningham M. Roelke D. Ingram |
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| Affiliation: | (1) Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, University of Georgia, Tifton, Georgia, 31793;(2) Florida Fish and Wildlife Conservation Commission, Gainesville, Florida;(3) Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee;(4) Developmental Serology Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, New York;(5) Western University of Health Sciences, College of Veterinary Medicine, Pamona, CA;(6) Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD, USA;(7) Present address: National Center for Environmental Assessment–Washington, DC Division, Environmental Protection Agency, Research Triangle Park, NC, USA |
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| Abstract: | This study documents the seroprevalence of feline immunodeficiency virus (FIV) and puma lentivirus (PLV) in free-ranging and captive Florida panthers (Puma concolor coryi) (n = 51) and translocated Texas cougars (P. concolor stanleyana) (n = 10) from 1985 to 1998. The sera were tested for anti-FIV antibodies by enzyme-linked immunosorbent assay (ELISA) and Western blot tests. The ELISAs were read kinetically (KELA) and the sera were retrospectively examined by PLV peptide ELISA. Eleven panthers and one cougar were positive by KELA; 4 panthers and 4 cougars were equivocal; 35 panthers and 5 cougars were negative; and 1 panther had no data. Seven of the 11 KELA-positive panthers were also positive by Western blot tests and all but one were positive by PLV peptide ELISA. Ten KELA-negative and Western blot-negative cats, were positive by PLV peptide ELISA. KELA results varied within cats from one sample period to the next, but PLV peptide ELISA results were consistent. Territorial sympatry and mating behaviour, noted from radiotelemetry location data on the cats, may have contributed to viral transmission between seropositive animals. These findings suggest that Florida panthers and the introduced Texas cougars have been exposed to FIV and/or PLV. |
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| Keywords: | cougar feline immunodeficiency virus FIV Florida panther KELA puma lentivirus PLV peptide ELISA |
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