Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda |
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| Authors: | W D Huse L Sastry S A Iverson A S Kang M Alting-Mees D R Burton S J Benkovic R A Lerner |
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| Affiliation: | Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037. |
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| Abstract: | A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies. |
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