复合菌固态发酵高蛋白饲料添加剂条件的优化

以黑曲霉菌、产朊假丝酵母和枯草芽孢杆菌为出发菌株，通过对固体发酵工艺条件和培养基优化的研究，获得了一条优化后的复合菌固态发酵高蛋白饲料添加剂生产工艺。研究表明以黑曲霉菌、产朊假丝酵母和枯草芽孢杆菌接人固体比例为4：3：3．固体接种量50％，发酵温度30℃～32℃，含水量50％，发酵初始pH4．6左右，发酵时间50h为发酵条件，以豆饼粉85％，（NH4）：SO40．8％。KH：PO40．2％。麸皮15％．硫酸铜和硫酸锰分别加入0．8‰为固体培养基，所产酸性蛋白酶活力最高为15426u／g，而获得最大酵母菌菌数为35亿．小肽含量为12．86％。粗蛋白含量为52．32％的高蛋白饲料添加剂。

Composite Bacteria of Solid State Fermentation Conditions of High Protein Feed Additive Optimization.

To black Aspergillus and Candida yeast and Bacillus subtilis as the starting strain, through the fermentation process to solid conditions and optimization of the medium, it was an optimization to a composite of solid state fermentation of high protein feed additive production process. Research shows that a black Aspergillus and Candida yeast and Bacillus subtilis access solid ratio of 4..3：3, solid vaccination of 50 %, the fermentation temperature 30℃-32℃, water content of 50%. the initial fermentation pH 4. 6 About 50h fermentation time for fermentation conditions,Soybean powder to 85%, （ NH4 ）：SO4 0. 8%, KH： PO4 0.2%, wheat bran 15%, copper sulfate and manganese sulfate respectively 0.8 % to to solid medium, it produced by a maximum of protease activity 15426 u / g, while it got the maximum number of yeast to 3.5 billion, small peptide content of 12. 86 %, the crude protein content of 52. 32 % of the high protein feed additive.