A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis |
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| Authors: | Aki Namba Yuya Shigenobu Masahiro Kobayashi Takanori Kobayashi Ichiro Oohara |
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| Affiliation: | (1) Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama Kanagawa, 236-8648, Japan;(2) Research Center for Fisheries and Environment in the Ariake and Yatsushiro Bays, Seikai National Fisheries Research Institute, Fisheries Research Agency, 1551-8 Taira-machi, Nagasaki Nagasaki, 851-2213, Japan;(3) Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa Kanagawa, 252-0880, Japan |
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| Abstract: | To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, β-proteobacteria, γ-proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group. |
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