Cloning and Sequence Analysis of Y-box Binding Protein Gene in Min Pig |
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| Authors: | Zhang Dongojie Liu Di Wang Liang He Xin-miao Wang Wen-tao |
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| Institution: | Institute of Animal husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| Abstract: | In order to study the gene sequence of Min pig Y-box binding protein (YB-1) gene, the complete coding sequence of Min pig YB-1 gene was cloned by RT-PCR, the sequence features were analyzed by some software and online website. The results showed that the complete CDS of Min pig Y-box was found to be 975 bp long, encoding 324 amino acids. It contained a conserved cold shock domain and several phosphorylation sites, but had no transmembrane domains, and was consistent with a protein found in the cytoplasm. Min pig YB-1 nucleotides shared high similarity (61.37%–97.66%) with other mammals. |
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| Keywords: | Min pig Y-box binding protein sequence feature |
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