龙葵果中原花青素优化提取试验研究 |
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引用本文: | 徐亚维,张新研,吕绍武,罗贵民.龙葵果中原花青素优化提取试验研究[J].现代农业科技,2010(23):12-14. |
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作者姓名: | 徐亚维 张新研 吕绍武 罗贵民 |
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作者单位: | 吉林农业科技学院生物工程学院;吉林大学生命科学学院;吉林大学分子酶学工程教育部重点实验室; |
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摘 要: | 以野生龙葵果为原料,以原花青素提取率为考察指标,研究提取溶剂浓度、料液比、提取温度、提取时间等因素对原花青素提取效果的影响。通过正交试验,确定野生龙葵果中原花青素的最佳提取工艺条件为:提取溶剂为60%的乙醇,料液比1∶15,提取温度25℃,提取时间80 min。此优化条件下提取野生龙葵果中原花青素的含量最高,达0.055 2%。
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关 键 词: | 龙葵果 原花青素 优化提取 |
Study on Optimal Extraction of Proanthocyanidin from Solanum nigrum Fruit |
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Authors: | XU Ya-wei ZHANG Xin-yan LV Shao-wu LUO Gui-min |
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Institution: | XU Ya-wei 1 ZHANG Xin-yan 2 LV Shao-wu 3 LUO Gui-min 3(1College of Bioengineering,Jilin Agricultural Science and Technology College,Jilin Jilin 132101,2School of Life Science and Technology,Jilin University,3 Key Laboratory for Molecular Enzymology and Enginnering of Ministry of Education,Jilin University) |
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Abstract: | Using wild Solanum nigrum fruit as raw materials,and extraction rate of proanthocyanidin as study index,effects of the concentration of solvent extraction,the ratio of material and fluid,extraction temperature and extraction time on extraction of proanthocyanidin were studied.Through orthogonal experiment,optimum extraction conditions were conclued as follows:extraction solvent was 60% ethanol,ratio of material to liquid was 1∶15,extraction temperature was 25 ℃,extraction time was 80 minutes.Under optimum conditions,the highest content of proanthocyanidins was obtained,reached 0.055 2%。 |
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Keywords: | Solanum nigrum fruit proanthocyanidins optimal extraction |
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