钙和钙调素拮抗剂对高温胁迫下茄子幼苗抗氧化系统的影响

 以耐热性不同的农友茄和二苠茄幼苗为材料，研究了钙、钙调素拮抗剂三氟拉嗪（TFP）和W7[N-（6-aminohexyl）-5-chloro-1-naphthalene sulfonamide]预处理对高温胁迫下（40℃/40℃，昼/夜）茄子幼苗丙二醛（MDA）、超氧阴离子（O2-·）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、谷胱甘肽（GSH）和脯氨酸（Pro）含量的影响。结果表明，随着高温胁迫时间的延长，TFP和W7浸种处理显著提高了茄子幼苗MDA含量和O2-·产生速率，同时，对SOD和POD活性产生抑制作用，加剧了GSH的破坏和Pro的积累；而Ca2+处理显著降低了高温胁迫下茄子幼苗MDA含量和O2-·产生速率，提高了SOD和POD活性，减轻GSH的破坏，延缓了Pro的积累。相同处理条件下，耐热品种农友茄较热敏感品种二苠茄伤害程度轻，这表明Ca2+-CaM信使系统可能通过提高抗氧化酶活性、调节抗氧化剂含量、降低膜质过氧化水平来调节茄子幼苗对高温逆境的适应性。

Effects of Calcium and Calmodulin Antagonist on Antioxidant Systems of Eggplant Seedlings Under High Temperature Stress

After being enhanced or blocked Ca2+-CaM signal transduction by pretreating the eggplants of 'Nongyouqie' and 'Erminqie' with external Ca2+ and calmodulin antagonists TFP and W7, production rate of O2-, the content of MDA, activities of SOD and POD and the contents of Pro and GSH under high temperature stress of 40℃(day/night)were investigated. The results showed that seeds were pretreated with calmodulin antagonist, TFP and W7 resulted in more rapid loss of SOD and POD activity, higher contents of MDA, Pro, and production rate of O2-, in addition, aggravate the damage of GSH; On the contrary, Ca2+ treatment enabled the seedlings to keep relatively high activities of SOD and POD, lower production rate of O2-and MDA content, at the same time, alleviated the damage of GSH and the accumulation of Pro caused by high temperature stress. These results indicated that Ca2+-CaM signal transduction might regulate the heat resistance of eggplant seedlings by affecting the activity of some antioxidant enzymes and the content of antioxidant substance.