| 一种虫草无性型营养缺陷型的诱变筛选 |
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| 引用本文： | 陈晓琳,樊美珍,等.一种虫草无性型营养缺陷型的诱变筛选[J].安徽农业大学学报,2002,29(2):166-168. |
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| 作者姓名： | 陈晓琳 樊美珍 |
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| 作者单位： | 安徽农业大学森林利用学院,合肥,230036 |
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| 摘 要： | 古尼虫草小孢变种(Cordyceps gunnii var.minor)的无性型古尼拟青霉小孢变种(Paecilomyces gunnii var.minor)G106菌株用两次紫外线处理法进行诱变,可使突变率增加4倍.对其中两株生长较好的突变株进行鉴定,结果为蛋氨酸缺陷型和亮氨酸缺陷型.稳定性检验结果表明这两株缺陷型均可作为原生质体融合时具有遗传标记的融合亲本.
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| 关 键 词： | 古尼拟青霉小孢变种 营养缺陷型 诱变 |
| 文章编号： | 1000-2197(2002)02-0166-03 |
| 修稿时间： | 2002年1月31日 |
Isolation of Auxotrophic Mutants of G106,a Strain of Paecilomyces gunnii var.minor,the Anamorph of Cordyceps gunnii var.minor |
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| CHEN Xiao lin,FAN Mei zhen,LI Zeng zhi.Isolation of Auxotrophic Mutants of G106,a Strain of Paecilomyces gunnii var.minor,the Anamorph of Cordyceps gunnii var.minor[J].Journal of Anhui Agricultural University,2002,29(2):166-168. |
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| Authors: | CHEN Xiao lin FAN Mei zhen LI Zeng zhi |
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| Abstract: | Conidium suspension of G106 was treated with UV twice,the mutation rate increased by 4 times Two mutant strains that grew better than others were selected and were identified as met - and leu - respectively. Stability experiments showed that the 2 strains were stable and were used as parents of genetic marker for proplast fusion. |
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