| 鸡肿瘤病料中马立克氏病病毒和禽网状内皮组织增生症病毒共感染的研究 |
| |
| 引用本文： | 张志,崔治中,姜世金,周蛟. 鸡肿瘤病料中马立克氏病病毒和禽网状内皮组织增生症病毒共感染的研究[J]. 中国预防兽医学报, 2003, 25(4): 274-278 |
| |
| 作者姓名： | 张志 崔治中 姜世金 周蛟 |
| |
| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018
2. 北京市农业科学院,畜牧兽医研究所,北京,100081 |
| |
| 基金项目： | 国家自然科学基金资助项目 ( #3 0 2 70 0 60 )，山东省科学技术发展计划 (鲁科计字 [2 0 0 2 ]3 87号 ) |
| |
| 摘 要： | 采用细胞培养、间接荧光抗体试验(IFA)、聚合酶链式反应(PCR)和斑点杂交(Dotblot)的方法从我国不同地区发生肿瘤的病料中同时进行MDV和REV的分离和鉴定。在分离到的13株MDV野毒株中，有4株培养物既能在IFA中与REV的单抗反应，又可以用PCR扩增出REV的LTR；另有4株培养物能扩增出REV的LTR，但在IFA中却不与REV的单抗反应。结果表明我国MD肿瘤中存在着REV的共感染，且我国MDV某些野毒株的基因组中有可能已经整合进了REV的LTR序列。
|
| 关 键 词： | 马立克氏病病毒 禽网状内皮组织增生症病病毒 共感染 |
| 文章编号： | 1008-0589(2003)04-0274-05 |
| 修稿时间： | 2002-11-26 |
Dual-infection of marke''''s disease virus and reticuloendotheliosis virus from tumors in chickens |
| |
| ZHANG Zhi ,CUI Zhi_Zhong ,JIANG Shi_jin ,ZHOU Jiao .Animal Science and Technology of Shan Dong Agricultural University,Taina ,China. Dual-infection of marke''''s disease virus and reticuloendotheliosis virus from tumors in chickens[J]. Chinese Journal of Preventive Veterinary Medicine, 2003, 25(4): 274-278 |
| |
| Authors: | ZHANG Zhi CUI Zhi_Zhong JIANG Shi_jin ZHOU Jiao .Animal Science Technology of Shan Dong Agricultural University Taina China |
| |
| Affiliation: | ZHANG Zhi 1,CUI Zhi_Zhong 1*,JIANG Shi_jin 1,ZHOU Jiao 2 1.Animal Science and Technology of Shan Dong Agricultural University,Taina 271018,China, 2.Institute of Animal Husbandry and Veterinary of Beijing 100081,China) |
| |
| Abstract: | Isolation and identification of Marek's Disease Virus(MDV) and Reticuloendotheliosis Virus(REV) were performed by cell culture,indirect fluorescence antibody assay(IFA),polymerase chain reaction(PCR)and dot blot from tumor samples in different regions of China.Among the 13 isolated MDV wild_type strains,there were 4 strains which showed positive reaction to monoclonal antibody of REV and PCR,and 4 strains showed negative with monoclonal antibody,but negative reaction to PCR.The results indicted that dual infection of MDV and REV was existed in tumors,and maybe the LTR sequence of REV had integrated into some wild_type MDV strains of China. |
| |
| Keywords: | Marker's disease virus reticuloendotheliosis virus dual_infection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
