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| 鹅细小病毒NS2基因的原核表达及抗原性检测 | |
| 引用本文： | 邢明伟,于天飞,马波,王君伟. 鹅细小病毒NS2基因的原核表达及抗原性检测[J]. 中国预防兽医学报, 2010, 32(2) |
| 作者姓名： | 邢明伟 于天飞 马波 王君伟 |
| 作者单位： | 1. 东北农业大学,动物医学学院,黑龙江,哈尔滨,150030;东北林业大学,野生动物资源学院,黑龙江,哈尔滨,150040 2. 东北农业大学,动物医学学院,黑龙江,哈尔滨,150030;齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔,161006 3. 东北农业大学,动物医学学院,黑龙江,哈尔滨,150030; |
| 基金项目： | 黑龙江"十五"攻关项目 |
| 摘 要： | 为原核表达鹅细小病毒(GPV)NS2蛋白,本研究利用特异性引物扩增获得GPV的H1分离株NS2基因,将其克隆于pMD18-T载体后进行序列测定。并将NS2基因亚克隆于原核表达载体pGEX-6P-1中,获得重组质粒pGEX-NS2。该质粒转化于感受态菌BL21(DE3)plysS中,经IPTG诱导,SDS-PAGE电泳分析,表达的重组蛋白约为75ku左右。经过亲和层析方法获得了纯化的重组NS2蛋白。Westernblot和Dot-ELISA鉴定结果表明,表达的重组NS2蛋白可以与GPV阳性血清发生特异性反应。 |
| 关 键 词： | 鹅细小病毒 NS2基因 原核表达 纯化 抗原性 |
Prokaryotic expression of goose parvovirus NS2 gene |
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| XING Ming-wei,YU Tian-fei,MA Bo,WANG Jun-wei. Prokaryotic expression of goose parvovirus NS2 gene[J]. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(2) | |
| Authors: | XING Ming-wei YU Tian-fei MA Bo WANG Jun-wei |
| Affiliation: | XING Ming-wei1,2,YU Tian-fei1,3,MA Bo1,WANG Jun-wei1(1. Department of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2. College of Wild Life Resources,Northeast Forestry University,Harbin 150040,3. College of Life Science , Engineering,Qiqihar University,Qiqihar 161006,China) |
| Abstract: | The goose parvovirus GPV NS2 gene was amplified by PCR and cloned into vector pGEX-6P-1. The recombinant plasmid pGEX-NS2 was transformed into BL21(DE3) pLysS E.coli and induced with IPTG. The expressed NS2 fusion protein had a MW of 75 ku and was purified by GST affinity chromatography purification system. Western blot and Dot-ELISA showed that the purified NS2 protein could be recognized by GPV positive serum. |
| Keywords: | GPV NS2 gene prokaryotic expression purification antigenicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |