骆驼刺高效液相指纹图谱研究

建立骆驼刺药材反相高效液相色谱指纹图谱分析方法，为有效控制骆驼刺药材的质量奠定基础。采用反相高效液相色谱，色谱柱为Waters Atlantis T3 C18（4.6 mm×250 mm，5 μm），用乙腈-0.2%甲酸水溶液线性梯度洗脱，以 1.0 mL·min^-1为流速，检测波长为 280 nm，采集时间为 100 min，分析了10批骆驼刺的HPLC指纹图谱。得到了分离度较好的骆驼刺药材高效液相色谱指纹图谱，标定出了11个共有峰，方法学考察结果符合指纹图谱技术要求。确定的指纹图谱研究方法稳定、可靠，可用于骆驼刺药材的质量分析。

HPLC Fingerprint Chromatogram of Alhagi sparsifolia Shap.

Alhagi sparsifolia Shap.is a perennial subshrub in the Leguminosae family.It grows mainly in Central Asia and Xinjiang Uygur Autonomous Region,China,and has been used in traditional Uygur medicine for a long time.It is widely used for treating physically weak,dizzy,cold,headache,intestinal constipation and arthrodynia.It mainly contains flavonoids,sterols, alkaloids, fatty acids and polysaccharides.There is no any report on the fingerprint chromatogram or chemical marker of A.sparsifolia yet.In order to develop the fingerprint chromatogram and provide the basis for the effective control of A.sparsifilia quality, the samples were separated by RP HPLC on a Waters Atlantis T3 C18 (4.6 mm×250 mm, 5 μm), with acetonitrile and 0.2% formic acid as mobile phase in a linear gradient manner.The flow rate was 1.0 mL·min^-1, the wavelength of detection was 280 nm, and the acquisition time was 100 min.HPLC fingerprint chromatograms of 10 batches of A.sparsifolia were analyzed.The HPLC fingerprint chromatogram of A.sparsifolia was developed with satisfactory resolution and 11 common fingerprint peaks with high similarity of correlation coefficients above 0.9.The peak No.10 and peak No.11 were the main compounds of A. sparsifolia, with the retention time matching isorhamnetin→3→O→β→D→galactosyl→(6→1) →α→L→rhamnoside and isorhamnetin→3→O→β→D rutinoside by HPLC, respectively. The results met the requirements of fingerprint technology. It was identified that the fingerprint method was stable and reliable, and could be used for the quality control of medicinal A. sparsifolia.