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陕北红枣中酚类物质的紫外光谱分析
引用本文:张向前,陈宗礼,杨选文,刘晓丽,张晓鑫.陕北红枣中酚类物质的紫外光谱分析[J].中国农学通报,2010,26(24):83-88.
作者姓名:张向前  陈宗礼  杨选文  刘晓丽  张晓鑫
作者单位:[1]延安大学生命科学学院,陕西延安716000 [2]陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000
基金项目:陕西省科学技术研究发展计划项目,陕西省教育厅专项科研计划项目,延安大学科研计划项目
摘    要:红枣样品经过超声波法、索氏回流法、萃取法三种不同方法处理后,采用紫外分光光度法,在相应检测波长下对没食子酸等9种酚类物质的含量进行测定。结果表明:除没食子酸外,儿茶素等八种酚类化合物检测浓度在0.002~0.010 mg/mL范围内与吸光度线性关系良好,相关系数(r2)为0.9954~0.9998,以儿茶素为对照品的平均回收率为99.30%,相对标准偏差(RSD)为1.87%。儿茶素和表儿茶素占检测到的酚类物质总量的55%以上。该方法简单、准确,可用于陕北红枣中酚类物质含量的测定。

关 键 词:桑叶  桑叶  γ-氨基丁酸  品种  比色法  含量  
收稿时间:2010/5/24 0:00:00
修稿时间:7/4/2010 12:00:00 AM

Analysis of Phenolic Compounds in North Shaanxi Red Jujube by UV Spectrophotometry
Zhang Xiangqian,Chen Zongli,Yang Xuanwen,Liu Xiaoli,Zhang Xiaoxin.Analysis of Phenolic Compounds in North Shaanxi Red Jujube by UV Spectrophotometry[J].Chinese Agricultural Science Bulletin,2010,26(24):83-88.
Authors:Zhang Xiangqian  Chen Zongli  Yang Xuanwen  Liu Xiaoli  Zhang Xiaoxin
Institution:1 College of Life Science, Yan ’ an university, Yan ’ an Shanxi 716000; 2 Shaanxi Research Center of Engineering TechnoLogy for Conservation and UtiLization of RegionaL BioLogicaL Resource, Yan ’ an Shanxi 716000)
Abstract:The phenolic compounds in north shaanxi red jujube were extracted by ultrasonic wave, Soxhlet backflow and extraction, respectively. Then the contents of phenolic compounds were detected by UV spectrophotometry at different wavelengths. The results showed that there were good linear relationships between eight phenolic compounds (except gallic acid) and their absorbance, when the concentrations of 8 phenolic compounds were from 0.002 to 0.010 mg/mL. The correlation coefficients ( r 2 ) were between 0.9954-0.9998, the average recovery was 99.30% and the RSD was 1.87% when catechin was as the control. The contents of catechin and epicatechin accounted for up to 55% . This method was simple, accurate and suitable for detecting phenolic compounds in north shaanxi red jujube.
Keywords:north shaanxi red jujube  phenolic compounds  UV spectrophotometry
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