樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性

采用菌丝生长速率法测定了樱桃褐腐病菌Monilinia fructicola对啶酰菌胺的敏感性,同时研究了不同敏感性菌株的生物学性状,探究了琥珀酸脱氢酶B亚基的氨基酸突变与其对啶酰菌胺产生抗性的相关性,并分析了樱桃褐腐病菌对啶酰菌胺与其他3种琥珀酸脱氢酶抑制剂(SDHIs)氯苯醚酰胺、氟唑菌苯胺和氟吡菌酰胺之间的交互抗...

Sensitivity of Monilinia fructicola to boscalid and cross-resistance to four succinate dehydrogenase inhibitors

The sensitivity of Monilinia fructicola to boscalid was determined by the mycelial growth rate method. Meanwhile, the study investigated the biological characteristics of isolates with different sensitivities, the correlation between the amino acid mutations of SDHB protein with resistance to boscalid, and the cross-resistance of M. fructicola between boscalid and other 3 tested succinate dehydrogenase inhibitors (SDHIs), Y12196, penflufen and fluopyram. The results showed that boscalid had a good inhibitory activity and therapeutic effect against M. fructicola. However, M. fructicola has developed a certain degree of resistance, and the resistant isolates have comparable fitness with the sensitive ones. The sequence analysis of SDHB gene showed that the amino acid point mutations of M. fructicola SDHB subunits were not significantly correlated with the resistance to boscalid. The cross-resistance studies indicated that there was cross-resistance between boscalid and the other three SDHIs, Y12196, penflufen, and fluopyram. In order to retard the occurrence and exacerbation of resistance, the rational mixes or rotations of boscalid with other kinds of fungicides rather than SDHIs are recommended during the prevention and control of cherry brown rot disease.