菊属植物远缘杂交亲和性研究

 开展了不同倍性菊属物种间远缘杂交试验，比较不同杂交组合的杂交亲和指数，并对授粉后生殖生物学过程（柱头花粉附着、花粉萌发、花粉管生长、受精及子房发育）进行荧光显微观察。结果表明，具相同倍性的种间及四倍体与六倍体物种间杂交较易结籽，而二倍体与四倍体或六倍体间难以结籽，表现杂交不亲和性。菊属远缘杂交不亲和表现为柱头上花粉粒粘附少，花粉萌发率低，花粉管生长异常难以进入花柱，诱导柱头胼胝质生成，受精胚败育。

Compatibility of Interspecific Cross in Dendranthema Genus

The interspecific cross was attempted between different species/cultivars of different ploidy in Dendranthema genus. The compatible index was calculated in different interspecific crosses. In addition, pollen adhesion, pollen germination, pollen tube growth, fertilization and ovary development were observed under fluoroscope. The results showed that seed-set was easily obtained from interspecific cross between the species of same ploidy or those between the tetraploid and hexaploid. However, few seed-set was obtained from the interpecific cross between diploid and tetrapoliod or hexaploid species, incompatibility was observed. The incompatibility of interspecific cross was characterized by low pollen adhesion, low pollen germination rate, abnormal pollen tube hampered extension on the stigma, calloses deposit on the stigma and collapsed ovary.