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| 旋毛虫基因重组抗原快速ELISA诊断试剂盒的研究 | |
| 引用本文： | 陈春花,李喜仙,阎玉河,邓瑞广. 旋毛虫基因重组抗原快速ELISA诊断试剂盒的研究[J]. 华北农学报, 2002, 17(2): 137-140 |
| 作者姓名： | 陈春花 李喜仙 阎玉河 邓瑞广 |
| 作者单位： | 1. 郑州牧业工程高等专科学校,河南,郑州,450008 2. 河南省新乡市新华区卫生防疫站,河南,新乡,453000 3. 河南省农业科学院生物技术研究所,河南,郑州,450002 |
| 摘 要： | 用旋毛虫基因重组抗原（TSRA）建立了诊断猪旋毛虫病的快速ELISA诊断试剂盒，用TSRA和旋毛虫肌幼虫排泄－分泌抗原（ES）分别对12份人工感染旋毛虫病猪血清进行快速ELISA检测，阳性率为100％。在猪旋行虫病发病区随机采集103份血清和肉样，分别用TSRA和ES作抗原对血清进行快速ELISA检测，结果显示，它们与肌肉消化法的诊断符合率分别为95．12％和96．3％，研究证明，用TSRA组装的诊断试剂盒具有良好的灵敏性，特异性和重复性。 |
| 关 键 词： | 旋行虫病 重组抗原 ELISA试剂盒 诊断试剂盒 猪 |
| 文章编号： | 1000-7091(2002)02-0137-04 |
| 修稿时间： | 2001-12-02 |
Study on an ELISA Kit with Recombinant Antigen for Rapid Diagnosis of Swine Trichinellosis |
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| CHEN Chun hua ,LI Xi xian ,YAN Yu he ,DENG Rui guang. Study on an ELISA Kit with Recombinant Antigen for Rapid Diagnosis of Swine Trichinellosis[J]. Acta Agriculturae Boreali-Sinica, 2002, 17(2): 137-140 | |
| Authors: | CHEN Chun hua LI Xi xian YAN Yu he DENG Rui guang |
| Affiliation: | CHEN Chun hua 1,LI Xi xian 2,YAN Yu he 3,DENG Rui guang 3 |
| Abstract: | An ELISA kit for rapid diagnosis of swine trichinosis was set up.Two antigens,gene recombinant antigen(TSRA)and ES antigen,were respectively used in the rapid ELISA to assay the blood samples of 12 pigs infected experimentally with Trichinella spiralis, the positive rates with both antigens were 100%.The serum and the meat samples of 103 pigs in an endemic regions were simultaneously collected and detected with TSRA and ES antigens for a compared test of digestive technique,the diagnostic agreement rates were 95 12% and 96 3%,respectively.It is shown that the kit is sensitive,specific and reproducible. |
| Keywords: | Swine trichinellosis Recombinant antigen ELISA kit |
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