皂化法提取胞内辅酶Q_(10)的研究

以根癌土壤杆菌为出发菌株,采用发酵法生产辅酶Q10,考察了破壁方法、皂化温度、皂化时间、加碱量、皂化顺序对辅酶Q10提取率的影响,试验得到皂化的最佳条件为:皂化温度60℃,皂化时间30min,皂化液与破碎液比例为5∶2。与未皂化相比,皂化后的细胞碎片经固液萃取后得到的辅酶Q10提取量提高了39.65%,并且消除了杂质对辅酶Q10测定的影响,该方法可行性强,具有广阔的发展前景。

Study on the Intracellular Coenzyme Q10 Extraction with Saponification Method

Using Agrobacterium tumefaciens as starting strain,producing Coenzyme Q10 by fermentation.The saponification method is applied to extracting Coenzyme Q10 in this paper,Influences of several factors such as cell-break method,saponification temperature and time,adding alkaline quantity,saponification sequence on Coenzyme Q10 extracting were studied.The best conditions of saponification were temperature 60℃,time 30min and saponification fluid∶breaking fluid=5∶2,After being saponification,the extracted amount of Coenzyme Q10 was about 39.65% higher than that not saponified,and eliminated the effect of impurity on determination of the content of Coenzyme Q10 in cell.The method has opened vast vistas.