稻属间杂种花粉植株的细胞遗传学研究

以栽培稻０２４２８与紧穗野生稻间不育Ｆ１杂种（２ｎ＝２４，ＡＣ）为材料进行花约培养，共接种花约３２６８８个，获得再生绿苗１３株及白化苗两株，愈伤诱导率与分化率分别为０．１４％和３４．７８％。有９朱花粉绿苗长至成熟，均表现长芒、紫柱头、强感光及易落粒等野生亲本的特性。细胞学结果进一步表明，其中４株为二倍体（２ｎ＝２４），形态上琚的Ｆ１杂种相似，花粉母细胞中期Ⅰ亦只能见到少数二价体；另５株则为四倍体（

Cytogenetical Studies on the Pollen-derived Plants from Interspecific Hybrid in Genus Oryza

Anther culture of a male-sterile interspecific hybrid between Oryza sativa vs. 02428 and O. eichingeri was attempted. From the 32688 anthers cultured, a total of 13 regenerated green plants and two albinos were eventually obtained, with the rate of callus induction and that of differentiation being 0. 14% and 34. 78%, respectively. All 9 green plants surviving to maturity showed traits of the wild species, including long awn, purple stigma, high photo-sensitivity and easy-shattering. They were further cytologically identified to be either diploids(2n = 24)or tetraploids(2n=48), in which 4 infertile diploids were similar to the original F1 hybrid morphologically and had also only a few bivalents at MI in PMCs, while two amphiploids with low self fertility and BPH resistance were, however, selected from the remained 5 tetraploid plants. It is therefore inferred that the F1 hybrid may have produced functional unreduced gametes from which the green plants originated.