| 食用菌过氧化物酶同工酶不同染色法效果比较 |
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| 引用本文： | 程东升,潘学仁.食用菌过氧化物酶同工酶不同染色法效果比较[J].中国食用菌,1994,13(3):15-18. |
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| 作者姓名： | 程东升 潘学仁 |
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| 作者单位： | 东北林业大学 |
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| 摘 要： | 过氧化物酶同工酶（ＰＯＤ）是真菌遗传分析中的一个常用生化指标，在其电泳分析中染色操作是一个重要技术环节。本研究以香菇、美味侧耳为材料，比较了不同ＰＯＤ染色方法的染色效果。ＮＨ＿３Ｃｌ－联苯胺法效果最佳，醋酸一联苯胺法次之，ＡＣ－联苯胺及愈创木酚－联苯胺法效果较差。对影响染色效果的原因进行了分析。
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| 关 键 词： | 食用菌，过氧化物酶同工酶，染色方法 |
Comparison of Staining methods for Analysing Peroxidase Isozyme of Edihle Fungi |
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| Cheng Dongsheng Pan Xueren.Comparison of Staining methods for Analysing Peroxidase Isozyme of Edihle Fungi[J].Edible Fungi of China,1994,13(3):15-18. |
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| Authors: | Cheng Dongsheng Pan Xueren |
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| Abstract: | Peroxidase isozymes (POD) are one of the frequently-used biochemical markers in genetical analysis of fungi,The staining is an important procedure in studying POD.Theefficiency was compared;among different stain recipes for POD of two edible fungi Lentinus edodes and Pleurctus sapidus. The recipe NH_3Cl-Benzidine showed the best effect,and followed the HAc-Benzidine,but the Vc-Benzidine and Guaiacol-Benzidine did not gave satisfactory results,The possible reasons for differences of stain effect were discussed. |
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| Keywords: | Edible fungi Peroxidase isozyme staining methods |
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