表面等离子共振生物传感器检测培氟沙星的方法研究

研究表面等离子共振生物传感器检测缓冲液中培氟沙星的方法，为样品的检测提供依据。采用抑制法，将培氟沙星与卵清蛋白的偶联物固定在芯片上，将抗体与不同浓度的培氟沙星混合后通入芯片表面，优化反应条件，构建标准曲线。结果显示，选择1∶1000稀释的抗体，流速7 μL/min，反应时间4 min，构建标准曲线，可以达到较低的检测限。利用SPR生物传感器建立快速检测缓冲液中培氟沙星的方法，检测限为0.8 ng/mL，检测时间为4 min，为样品检测奠定基础。

The Methods of Detecting Pefloxacin by Surface Plasmon Resonance Biosensor

This experiment is to establish methods of detecting pefloxacin in HBS-EP buffer by SPR biosensor, which can provide a basis for sample detection. The pefloxacin-ovalbumin conjugate was covalently immobilized on CM5 chip and the antibody was mixed with pefloxacin of different concentrations to construct a standard curve. To obtain lower limit detection, the diluted antibody of 1000 times was injected at a flow rate of 7 μL/min for 4 min. It was shown from the experimental results that a rapid detection of pefloxacin in buffer using SPR biosensor had been established and the limit of detection was 0.8 ng/mL, the detection time was 4 min.