| 鸡免疫球蛋白(GIgG)轻链的分离纯化及其抗血清的制备 |
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| 引用本文： | 李庆章,刘忠贵.鸡免疫球蛋白(GIgG)轻链的分离纯化及其抗血清的制备[J].中国兽医学报,1989(1). |
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| 作者姓名： | 李庆章 刘忠贵 |
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| 作者单位： | 东北农学院兽医系
(李庆章)，东北农学院兽医系(刘忠贵) |
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| 摘 要： | 经硫酸铵盐析、柱色谱分离得到纯化的鸡血清IgG。继用2-巯基乙醇还原、碘乙酰胶烷化拆开IgG的轻、重链,再经Sephadex G100凝胶过滤柱色谱分离得到IgG轻链。以IgG轻链免疫家兔制得兔抗鸡IgG轻链抗血清。
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| 关 键 词： | 鸡 IgG IgG轻链 家兔 抗血清 |
Isolation and Purification of Chicken Immunoglobulin G (IgG)'Light Chain and Preparation of Its Antisera |
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| Li Qingzhang Veterinary.Isolation and Purification of Chicken Immunoglobulin G (IgG)'Light Chain and Preparation of Its Antisera[J].Chinese Journal of Veterinary Science,1989(1). |
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| Authors: | Li Qingzhang Veterinary |
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| Abstract: | Immunoglobulin G(IgG ) in chicken serum was obtained bysalting-out of ammonium sulfate and isolated on column chromatography.The reduction and alkylation of the IgG were performed in Tris bufferusing 2-mercaptoethanol and iodoacetamide. Light chain of IgG was separatedby Sephadex G 100 gel filtration. The antisera of rabbit anti-chicken IgGIight chain was prepared. |
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| Keywords: | chicken IgG IgG light chain rabbit antisera |
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