籼、粳型转基因水稻花粉源基因飘流差异研究

[目的]在同等条件下研究比较籼、粳型转基因水稻花粉源基因飘流差异,为控制转基因水稻基因飘流提供参考.[方法]以籼型转基因水稻Bar9311和粳型转基因水稻B2为花粉供体材料,常规籼稻特籼占25和籼型不育系博A为受体材料,在自然条件下研究籼、粳型转基因水稻向不育系和常规稻的基因飘流频率.[结果]当受体为不育系时,无论花粉源是籼型还是粳型转基因水稻,在不同距离点的基因飘流频率均为粳型>籼型.10m试验区内籼型转基因水稻向不育系各点平均基因飘流频率为1.325%~23.948%,粳型为3.906%~45.934%;而当受体为常规稻时,籼型转基因水稻向常规稻各点平均基因飘流频率为0.015%~1.189%,粳型为0.041%~0.435%.此外,随着距离的变化,在6m内的基因飘流频率是粳型<籼型,之后两者出现交互现象.无论籼型还是粳型转基因水稻,向不育系基因飘流的频率远高于向常规稻的飘流频率,且基因飘流频率均随与花粉源距离的递增而递减.[结论]不同类型的花粉供体或受体,都会对基因飘流频率产生影响.

Comparison of gene flow from transgenic Indica and Japonica rice pollen donor

【Objective】Difference in gene flow between transgenic Indica and Japonica rice under the same condition was investigated to provide reference for controlling gene flow of transgenic rice. 【Method】Taking Indica transgenic rice Bar9311 and Japonica transgenic rice B2 as pollen donors， conventional rice Texianzhan25 and male sterile line BoA as pollen receptors， gene flow frequencies from Indica and Japonica transgenic rice to conventional rice and male sterile line were studied by using cross-pollination with the wind in 10 m. 【Result】While taking male sterile line as pollen receptor， irrespective of pollen donor type， the frequencies of transgene flow （FTF） in Japonica transgenic rice to MS line in different distance were higher than Indica transgenic rice. The frequencies of FTF in Indica and Japonica transgenic rice to MS line within 10 m distance were 1.325-23.948% and 3.906-45.934%， respectively. While taking conventional rice as receptor， the FTFs from Indica and Japonica transgenic rice were 0.015-1.189% and 0.041-0.435%， respectively. In addition， within 6 m of distance， the FTF from Japonica transgenic rice was higher than that from the Indica transgenic rice at any distance， followed by the two FTFs intersecing over 6 m. The FTF of Indica and Japonica transgenic rice to MS line was much higher than that of conventional rice， and the FTFs showed descending trend with the increase of distance from pollen source. 【Conclusion】FTF was affected by different pollen donors or receptors.