新疆棉花黄萎病菌病原种群监测研究

采用致病性测定、营养亲和群测定及PCR特异性扩增3种方法,对新疆棉区采集的35个棉花黄萎病菌代表性菌系进行致病型鉴定。结果表明,新疆棉花黄萎病菌存在强、中、弱3种不同的致病类型,其中以中等致病类型居多。利用营养亲和群方法将新疆棉花黄萎病菌分为2个亲和群,其中1个亲和群中的所有菌系均与标准落叶型菌系相亲和,另1个亲和群中的所有菌系均与标准非落叶型菌系相亲和。利用棉花黄萎病菌落叶型特异引物进行PCR特异性扩增,有3个菌系扩增出550bp的落叶型黄萎病菌特异性片段。用营养亲和群及PCR特异性扩增均进一步证实目前新疆存在落叶型黄萎病菌菌系。

Population monitoring study of Verticillium dahliae in cotton in Xinjiang

The pathogenicity identification of 35 representative strains of Verticillium dahliae collected from the cotton-planted region in Xinjiang was studied using the methods of pathogenicity identification,vegetative compatibility group and PCR specific amplification.The results show:(1) the strains of Verticillium dahliae in Xinjiang can be separated into three groups,namely strong,middle and weak.Among them,most of the strains belong to the middle group.(2) the strains of Verticillium dahliae in Xinjiang can be separated into two vegetative compatibility groups.All strains in one vegetative compatibility group can be fused with standard defoliating pathotype and all strains in the other vegetative compatibility group can be fused with standard nondefoliating pathotype.This result makes sure the presence of the defoliating pathotype strains of Verticillium dahliae in cotton.(3) The result of PCR amplification using the specific primer of defoliating pathotype of Verticillium dahliae in cotton shows that the specific fragment (550 bp) of defoliating pathotype of Verticillium dahliae can be produced from three strains,which proves the presence of the defoliating pathotype strains of Verticillium dahliae in cotton in Xinjiang at present under the molecular level.