| M、F型COII基因在雌雄三角帆蚌不同发育时期的表达差异研究 |
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| 引用本文： | 陈亚 上海海洋大学农业部淡水水产种质资源重点实验室,王雅昱,汪桂玲,何方殊,李家乐. M、F型COII基因在雌雄三角帆蚌不同发育时期的表达差异研究[J]. 水产学报, 2018, 42(1): 29-38 |
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| 作者姓名： | 陈亚 上海海洋大学农业部淡水水产种质资源重点实验室 王雅昱 汪桂玲 何方殊 李家乐 |
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| 作者单位： | 上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306,上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306,上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306;上海市水产养殖工程技术研究中心, 上海 201306,上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306,上海海洋大学农业部淡水水产种质资源重点实验室, 上海 201306;上海市水产养殖工程技术研究中心, 上海 201306 |
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| 基金项目： | 国家自然科学基金（31772835）；上海市教委科研创新重点项目（13ZZ128）；上海高校知识服务平台项目（ZF1206） |
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| 摘 要： | 与传统的母系遗传不同,双壳贝类线粒体有M和F型2种mt DNA,称为双单亲遗传(doubly uniparental inheritance,DUI)。为了探究DUI的形成机制,实验利用RACE方法得到三角帆蚌M、F-type COⅡ基因的cDNA全长;荧光定量检测各时期各组织中M、F-type COⅡ基因的表达。结果发现,(1)M-type COⅡ基因的cDNA序列全长1244 bp,F-type COⅡ基因的cDNA序列全长808 bp,M型3′端较F型有一段546 bp的特异性延长序列;跨膜结构预测显示M-type COⅡ基因3′特异性延伸区域有4个跨膜结构,推测这段延伸序列具有一定的功能性;(2)早期幼龄(胚胎期-5月龄)三角帆蚌的组织团中,M、F-type COⅡ基因均能检测出,且同时期中F-type COⅡ基因的表达量显著高于M-type COⅡ;(3)6月龄蚌各组织中,F-type COⅡ基因在各组织中表达量明显高于M型的表达量,而M-type COⅡ基因在性腺中表达明显高于其他组织;(4)一龄雌性各组织中,M、F-type COⅡ基因均有表达;而一龄雄性各组织中,M-type COⅡ基因仅在性腺中表达;(5)二龄雌性各组织中,M、F-type COⅡ基因均有表达;二龄雄性各组织中,F-type COⅡ基因除性腺外的其他组织中均有低量表达,M-type COⅡ基因仅在性腺中表达,且表达量较高。研究表明,在三角帆蚌雌雄个体发育过程中,M-type mt DNA在组织中选择性降解或聚集。
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| 关 键 词： | 三角帆蚌 双单亲遗传 COⅡ基因 表达差异 |
| 收稿时间： | 2016-12-15 |
| 修稿时间： | 2017-03-29 |
Full-length cDNA cloning of M and F-type COII genes and expression in different ages freshwater mussel Hyriopsis cumingii |
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| CHEN YA,WANG YAYU,WANG GUILING,HE FANGSHU and LI JIALE. Full-length cDNA cloning of M and F-type COII genes and expression in different ages freshwater mussel Hyriopsis cumingii[J]. Journal of Fisheries of China, 2018, 42(1): 29-38 |
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| Authors: | CHEN YA WANG YAYU WANG GUILING HE FANGSHU LI JIALE |
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| Affiliation: | Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China,Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China and Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China |
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| Abstract: | Several species of molluscan bivalves have a peculiar mode of mtDNA transmission, known as doubly uniparental inheritance (DUI). In these species, two mitochondrial genomes, M-type mtDNA and F-type genomes, coexist in stable state. In this study, the cDNA sequence of M and F-type COII gene of H.cumingii was cloned by RACE, QRT-PCR was used to study the expression of M and F -type COII genes in H.cumingii at different stages. We found that (i) The full-length cDNA sequence of the M-type is 1244 bp; and F-type COII is 808 bp, the C-terminus extension to the M-type COII protein has four transmembrane regions confirming that the extension is functional (ii) M-type COII gene and F-type COII gene were all expressed in gonad tissues from embryos to 5-month-age old H.cumingii, and the expression level of F-type COII gene in the same period was significantly higher than that of M-type COII gene. (iii) In semi-age old H.cumingii, F-type mtDNA was expressed mainly in somatic tissues and little in gonads, while M type expressed mainly in gonads and little in somatic tissues; (iv) In one-age old H.cumingii, F and M type both expressed in female somatic tissues and gonads, while in males F type expressed in all tissues and M type only expressed in gonads; (v) In two-age old H.cumingii, F type was |
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| Keywords: | Hyriopsis cumingii Doubly Uniparental Inheritance COII gene expression differences |
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