| 棉花黄萎病菌田间动态的分子监测 |
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| 引用本文： | 王云月 Multa.,D.棉花黄萎病菌田间动态的分子监测[J].云南农业大学学报,1998,13(1):43-48. |
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| 作者姓名： | 王云月 Multa. D |
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| 作者单位： | 云南农业大学云南省植物病理重点实验室，澳大利亚悉尼大学生命科学学院分子遗传学实验室 |
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| 基金项目： | 澳大利亚国家自然科学基金 |
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| 摘 要： | 分别于1994年3月和1995年3月从同一棉田,采集分离了48个大丽轮枝菌菌株.应用RAPD技术,从240个随机引物中筛选了17个扩增效果好的引物,对供试菌株进行了DNA指纹分析.结果表明:17个引物对48个菌株扩增出120条DNA分子片段,其中43条分子片段为多态带,占35.83%.将扩增结果输入计算机,用PHYLIP分析软件(3.5版本),UPGAME程序进行类聚分析,结果表明95年的5个菌株与其它菌株存在明显的遗传差异.
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| 关 键 词： | 棉花黄萎病 RAPD DNA指纹 |
Molecular Detection of Verticillium dahliae Strains in Field |
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| Wang Yunyue,Zhu Youyong,Dilbag S Multani,Bruce R. Lyon.Molecular Detection of Verticillium dahliae Strains in Field[J].Journal of Yunnan Agricultural University,1998,13(1):43-48. |
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| Authors: | Wang Yunyue Zhu Youyong Dilbag S Multani Bruce R Lyon |
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| Institution: | Wang Yunyue 1 Zhu Youyong 1 Dilbag S Multani 2 Bruce R. Lyon 2 |
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| Abstract: | Forty eight isolates of Verticillium dahaliae have been collected and isolated from same cotton field in March, 1994 and March, 1995 respectively. These isolates were analyzed using RAPD fingerprint technique. The results showed that a total of 120 reproducible RAPD bands were obtained on 48 isolates with 17 random primers selected from 260 primers. Forty three of the 120 bands ( 35.83%) were polymorphic. When RAPD bands were used for cluster analysis using the PHYLIP 3.5 c UPGAME program, all isolates were clustered into 4 RAPD groups, and the genetic difference of RG4 containing 5 isolates of 1995 are very different from others. |
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| Keywords: | Verticillium dahliae RAPD Genetic difference |
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