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山羊胚胎体外培养条件的优化
引用本文:刘羿羿,赵高平,李燕,白俊,王申元,巴特尔,周欢敏. 山羊胚胎体外培养条件的优化[J]. 中国畜牧兽医, 2010, 37(4): 146-149
作者姓名:刘羿羿  赵高平  李燕  白俊  王申元  巴特尔  周欢敏
作者单位:(1.内蒙古农业大学生物工程学院, 呼和浩特 010018; 2.内蒙古农业大学动物科学学院, 呼和浩特 010018;3.内蒙古农业大学科技处, 呼和浩特 010018;4.内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室, 呼和浩特 010021)
基金项目:国家自然基金(30460091)
摘    要:为优化山羊胚胎体外培养条件,本试验比较了含有3种不同来源血清(优质胎牛血清、发情山羊血清、前列腺素(PG)处理后发情山羊血清)的M199对卵母细胞成熟效果的影响,成熟率分别为65.95%、49.2%、76.47%,差异极显著(P<0.01);本试验还分别采用了SOFaa、CR1aa+输卵管上皮细胞共培养,以及改进的DMEM/F12发育体系培养受精卵,结果发现,SOFaa、CR1aa+输卵管上皮细胞的发育培养系统得到的囊胚率为29.62%、24.73%,差异不显著(P>0.05),改进的DMEM/F12发育液囊胚率最高为48.42%,差异极显著(P<0.01)。

关 键 词:发情山羊血清  囊胚率  输卵管上皮细胞  

Optimization of Goat Embryos in vitro Culture Conditions
LIU Yi-yi,ZHAO Gao-ping,LI Yan,BAI Jun,WANG Shen-yuan,BAR Ter,ZHOU Huan-min. Optimization of Goat Embryos in vitro Culture Conditions[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(4): 146-149
Authors:LIU Yi-yi  ZHAO Gao-ping  LI Yan  BAI Jun  WANG Shen-yuan  BAR Ter  ZHOU Huan-min
Affiliation:(1.College of Biotechnology, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science and Medical Science, Inner Mongolia Agricultural University, Hohhot 010018, China;3.Science and Technology Agency, Inner Mongolia Agricultural University, Hohhot 010018, China;4.The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot 010021, China)
Abstract:This experiment compared the effect of mature liquid that containing three different sources serum: high-quality fetal bovine serum,estrus goat serum,PG treated estrus goat serum,maturation rate were 65.95%,49.2%,76.47%(P<0.01)respectively,the difference was extremely significant;compared SOFaa,CR1aa+ oviduct epithelial cells,and improved DMEM/F12 development system and found that,the blastocyst rate of SOFaa,CR1aa+ oviduct epithelial cells were 29.62%,24.73%,difference was not significant(P>0.05),the DMEM/...
Keywords:estrus goat serum  blastocyst rate  oviduct epithelial cells  
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