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龙眼品种(系)遗传多样性及亲缘关系的AFLP分析
引用本文:易干军,谭卫萍,霍合强,张秋明,李建光,周碧容.龙眼品种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报,2003,30(3):272-276.
作者姓名:易干军  谭卫萍  霍合强  张秋明  李建光  周碧容
作者单位:(广东省农业科学院果树研究所,广州 510640; 湖南农业大学,长沙 410128)
基金项目:广东省国际科技合作项目
摘    要: 选用两对引物组合EcoRⅠ ACT+MseⅠ CTT和 EcoRⅠ ACT+MseⅠ CTC对46份龙眼材料进行AFLP分析,共得到扩增位点111个,其中多态性位点103个,多态性比例达92.69%,区分率达100%。对AFLP扩增结果进行聚类分析,根据相似系数0.76的水平可将46份龙眼品种(系)分为11个品种群。供试各品种多态性比例介于0.2703和0.4955间。福建东壁与广东东壁应为不同品系;早熟一号为一个新的品系。

关 键 词:龙眼  AFLP  遗传多样性  亲缘关系
文章编号:0513-353X(2003)03-0272-05
收稿时间:2002-12-11
修稿时间:2002年12月11

Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP Analysis
Yi Ganjun ,Tan Weiping ,Huo Heqiang ,Zhang Qiuming ,Li Jianguang ,and Zhou Birong.Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP Analysis[J].Acta Horticulturae Sinica,2003,30(3):272-276.
Authors:Yi Ganjun  Tan Weiping  Huo Heqiang  Zhang Qiuming  Li Jianguang  and Zhou Birong
Institution:
( Institute of Fruit Tree Research,Guangdong Academy of Agricaltural Sciences, Guangzhou 510640,China; Hunan Agricultural University,Changsha 410128,China)
Abstract:The study is mainly on the genetic diversity and relationship of Longan (Dimocarpus longan Lour.) cultivars by applying AFLP (Amplified Fragment Length Polymorphism). 103 polymorphic distinct bands were obtained with two pairs of primers(EcoRⅠACT+Mse ⅠCTT and EcoR ⅠACT+Mse Ⅰ CTC),which were enough to distinguish the 46 cultivars from each other. UPGMA analysis showed that 46 cultivars were divided into 11 groups.The ratio of polymorphic rate varied from 0.2703 to 0.4955.The result showed that the Fujian Dongbi was diferent from Guangdong Dongbi and they were different types from Kaiyuansi Dongbi.Zaoshu 1 was supposed to be a new type.The results supplied theoretical basis for cultivar classification.
Keywords:Longan  AFLP  Genetic diversity  Relationship
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