| 水稻白叶枯病黄单胞菌赖氨酰－tRNA合成酶基因的定位、克隆及分析 |
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| 引用本文： | 奚传武,冯家勋,唐纪良. 水稻白叶枯病黄单胞菌赖氨酰－tRNA合成酶基因的定位、克隆及分析[J]. 广西农业生物科学, 1996, 0(1) |
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| 作者姓名： | 奚传武 冯家勋 唐纪良 |
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| 作者单位： | 广西农业大学实验中心分子遗传学研究室 |
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| 基金项目： | 国家863高技术计划项目 |
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| 摘 要： | 以３２Ｐ标记的甘蓝黑腐病黄单胞菌赖氨酰－ｔＲＮＡ合成酶基因为探针，将水稻白叶枯病黄单胞菌同功的基因定位于ｐＧＸＮ３０００１．６ｋｂ的ＢａｍＨＩ片段上．通过ＤＮＡ－ＤＮＡ杂交，标记置换等方法初步证实：在稻白叶枯病黄单胞菌中只有一个拷贝的赖氨酰－ｔＲＮＡ合成酶基因，该基因失活对病菌是致死的。
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| 关 键 词： | 黄单胞菌属；赖氨酰－tRNA合成酶；标记置换；致死突变 |
Location, Cloning and Analysis of Lysyl-tRNA Synthetase Gene of Xanthomonas oryzae pv. oryzae |
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| Xi Chuanwu, Feng Jiaxun, Tang Jiliang. Location, Cloning and Analysis of Lysyl-tRNA Synthetase Gene of Xanthomonas oryzae pv. oryzae[J]. Journal of Guangxi Agricultural and Biological Science, 1996, 0(1) |
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| Authors: | Xi Chuanwu Feng Jiaxun Tang Jiliang |
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| Abstract: | A lysyl-tRNA synthetase gene of X. o. pv. oryzae was located on the 1. 6 kb BamH I fragment of plasmid pGXN3000 by DNA-DNA hybridization using 32P-labelled lysS of X. c. pv. campestris and cloned in the vector pBR322. The results of DNA-DNA hybridization and Tn5 mutagenesis analysis show that there is only one copy of lysyl-tRNA gene in Xoo genome and mutation of the gene is fatal to Xoo. |
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| Keywords: | Xanthomonas Lysyl-tRNA synthetase marker exchange lethal mutation |
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