| 牦牛生长激素基因分子特性分析 |
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| 引用本文： | 王磊,吴建平,杨联,张利平,徐建峰,方翟.牦牛生长激素基因分子特性分析[J].甘肃农业大学学报,2009,44(6). |
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| 作者姓名： | 王磊 吴建平 杨联 张利平 徐建峰 方翟 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省科技攻关项目，教育部春晖计划 |
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| 摘 要： | 设计特异性引物,利用RT-PCR方法从牦牛脑垂体中扩增出生长激素基因(YGH)编码区全序列,并进行分子特性分析.结果表明:牦牛生长激素基因编码区全长654 bp,包含一个完整的阅读框,编码217个氨基酸.采用生物信息学方法对分子特性进行分析表明:动物生长激素基因在进化上非常保守,牦牛生长激素基因氨基酸序列与黄牛、山羊、绵羊、马、猪、猫和犬的同源性分别为100%、99.1%、98.6%、88.9%8、8.5%、88.9%和89.4%.牦牛生长激素蛋白有2个明显的疏水区域,存在2个强跨膜区和信号肽,是一种分泌型蛋白质,二级结构主要以α-螺旋和不规则卷曲为主,三级结构包含第28-215位氨基酸残基;牦牛生长激素蛋白有较高的抗原指数和抗原表位,这与YGH编码蛋白具有良好的抗原性相一致.
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| 关 键 词： | 牦牛 生长激素基因 克隆 分子特性 |
Molecular characteristics of growth hormone gene in yak |
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| WANG Lei,WU Jian-ping,YANG Lian,ZHANG Li-ping,XU Jian-feng,FANG Di.Molecular characteristics of growth hormone gene in yak[J].Journal of Gansu Agricultural University,2009,44(6). |
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| Authors: | WANG Lei WU Jian-ping YANG Lian ZHANG Li-ping XU Jian-feng FANG Di |
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| Institution: | WANG Lei,WU Jian-ping,YANG Lian,ZHANG Li-ping,XU Jian-feng,FANG Di(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | Growth hormone is a single-chain polypeptide hormone and secreted by anterior pituitary.GH exist in a wide variety of vertebrates,it can promote animal growth and development.The objective of this study was to clone the yak GH gene and do sequence analysis.A pair of specific PCR primers were designed for cloning the coding sequence of GH gene from pituitary tissue in yak.A fragment with 654 bp was got by RT-PCR amplifying,in which included a complete open reading frame and it encoded 217 amino acids(GeneBan... |
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| Keywords: | yak growth hormone gene cloning molecular characteristics |
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