不同分割液对奶牛不同阶段胚胎分割效果的影响

本试验探讨了3种不同分割液对奶牛桑葚胚和囊胚分割效果的影响。借助显微操作仪,将发育至第6～8天的体内常规生产的桑葚胚和囊胚进行分割,体外培养半胚,观察其发育情况,选择形态恢复好的半胚与一个囊胚滋养层细胞囊泡（trophoblastic vesicles,TRV）共移植。结果显示,在PBS+0.2 mol/L蔗糖与PBS+5%PVP中分割桑葚胚,其分割成功率显著高于PBS（P<0.05）,分别为89.13%、86.73%和69.67%,而半胚的囊胚发育率及移植妊娠率三者均无显著差异（P>0.05）;在PBS+0.2 mol/L蔗糖与PBS+5%PVP中分割囊胚, 其分割成功率显著高于PBS（P<0.05）,分别为94.52%、92.52%和70.52%,而半胚培养的囊胚发育率及移植妊娠率三者均无显著差异（P>0.05）;说明在PBS中分别添加0.2 mol/L的蔗糖和5%的PVP有利于提高奶牛桑葚胚和囊胚的分割成功率。

Effects of Different Partition Liquid on Dairy Cow Embryos Segmentation at Different Stages

In this experiment,morula and blastocyst were bisected in three different partition liquid with micromanipulator.Demi-embryos were cultured and then the development rate was observed.Bisected embryos were co-transferred with trophoblastic vesicles(TRV).The results showed that morula were bisected in PBS +0.2 mol/L sucrose and PBS+5% PVP,splitting success rate significantly higher than in PBS(89.13%,86.73% VS 69.67%)(P<0.05),and the blastocysts rate and pregnancy rate were no significant difference(P>0.05);i...