| 新疆艾比湖野鸟H1N2亚型禽流感病毒的分离鉴定及全基因组序列分析 |
| |
| 引用本文： | 李培东,刘庆庆,肖陈城,陈创夫,王丹,依再提古丽·热依木江,王一帆,朱雅,王勇,李亚玲.新疆艾比湖野鸟H1N2亚型禽流感病毒的分离鉴定及全基因组序列分析[J].中国兽医学报,2021(1):38-42,49. |
| |
| 作者姓名： | 李培东 刘庆庆 肖陈城 陈创夫 王丹 依再提古丽·热依木江 王一帆 朱雅 王勇 李亚玲 |
| |
| 作者单位： | ;1.石河子大学动物科技学院;2.第八师玛纳斯河湿地自然保护区管理站 |
| |
| 基金项目： | 石河子大学青年创新人才培育计划资助项目(CXRC201809);石河子大学高层次人才科研启动基金资助项目(RCSX201703,RCSX201702);国家自然科学基金资助项目(31702213);新疆生产建设兵团“2011协同创新中心”基金资助项目。 |
| |
| 摘 要： | 旨在调查野鸟中存在的禽流感病毒,并为禽流感的防制提供依据。采集新疆艾比湖自然保护区野鸟的粪便棉拭子进行禽流感病毒的分离与鉴定,将PCR产物进行纯化后用T载体连接并转化到DH5α细胞中;菌液PCR鉴定后将目的菌液送至上海生工测序并比对测序结果,在NCBI上进行基因序列分析。结果显示,从新疆艾比湖自然保护区野鸟的粪便棉拭子分离得到1株H1N2亚型禽流感病毒;经序列分析得知,该毒株为1株低致病性禽流感病毒,且存在与多种亚型病毒的重配现象。
|
| 关 键 词： | 新疆艾比湖 野鸟 H1N2亚型禽流感病毒 分离鉴定 序列分析 |
Isolation,identification and genome sequence analysis of wild bird H1N2 subtype avian influenza virus in Ebinur Lake,Xinjiang |
| |
| LI Peidong,LIU Qinqin,XIAO Chencheng,CHEN Chuangfu,WANG Dan,YIZAITIGULI Reyimujiang,WANG Yifan,ZHU Ya,WANG Yong,LI Yaling.Isolation,identification and genome sequence analysis of wild bird H1N2 subtype avian influenza virus in Ebinur Lake,Xinjiang[J].Chinese Journal of Veterinary Science,2021(1):38-42,49. |
| |
| Authors: | LI Peidong LIU Qinqin XIAO Chencheng CHEN Chuangfu WANG Dan YIZAITIGULI Reyimujiang WANG Yifan ZHU Ya WANG Yong LI Yaling |
| |
| Institution: | (College of Animal Science and Technology iShihezi University,Shihezi,Xinjiang 832000,China;Eighth Division Manas River Wetland Nature Reserve Management Station,Shihezi,Xinjiang 832000,China) |
| |
| Abstract: | The purpose of this study is to investigate the existence of avian influenza virus in wild birds and provide a basis for the prevention and control of avian influenza.The fecal cotton swabs of wild birds were collected from Ebinur Nature Reserve in Xinjiang to isolate and identify the virus.The viral RNA was extracted for RT-PCR amplification.The PCR products were purified,and linked with T-vector and transformed into E.coli DH5αcells.The transformants were selected on ampicillin-containing plates.After PCR identification,the bacterial fluid was sent to Shanghai Sangon Biological Engineering Technology and Services Co.Ltd for sequencing,and the sequence was analyzed in BLAST database of NCBI.The results showed that a strain of H1 N2 subtype influenza virus was isolated.Sequence analysis showed that the strain was a low pathogenic avian influenza virus which has the phenomenon of recombination with many subtypes. |
| |
| Keywords: | Ebinur Lake in Xinjiang wild birds H1N2 subtype avian influenza virus isolation and identification sequence analysis |
| 本文献已被 维普 等数据库收录！ |
|
