饲料添加黄金虫虫粉对小鼠肠道炎症相关细胞因子表达的影响

为研究日粮中添加不同比例黄金虫虫粉对动物肠道健康及局部炎症的影响,选取昆明小鼠28只,随机分为4组,各组基础日粮分别添加0％,5％,10％,20％黄金虫虫粉,饲喂2周后,灌胃0.05 mL/g的1× 108 CFU/mL的沙门菌菌液建立腹泻模型,荧光定量PCR检测小鼠肠道不同部位炎性因子及抗菌肽的表达量.结果显示,灌胃...

Effect of black soldier fly powder feed addition on intestinal inflammation-related cytokines in mice

To study the effects of adding different proportions of the black soldier fly powder to the diet on the intestinal health and local inflammation of animals,28 Kunming mice were randomly divided into 4 groups,the basic diet of each group was supplemented with 0%,5%,10%,and 20%of black soldier fly powder.Two weeks later,1×10⁸CFU of Salmonella was administered orally to establish a diarrhea model,and the expression of inflammatory factors and antibacterial peptides in different parts of the mouse intestine were detected by qRT-PCR.The results showed that after gavage,the expressions of proinflammatory factors TNF-αand IL-6 in the jejunum,ileum and colon of mice fed with different proportions of black soldier fly powder were significantly lower than those in the control group,the expression of anti-inflammatory factors IL-4,IL-10,and IL-13 were significantly increased,and the expression of mouse-derived antibacterial peptides Ang-4,Cryptdin-1,Cryptdin-5,Lyz2,Crs1 c,and Reg3 r were significantly increased too.These results indicated that the black soldier fly powder as a feed additive can improve the immunity of mice,and increase the resistance of intestine to pathogens and inhibit the inflammatory response when Salmonella invades the intestine.