细胞种类对阳离子脂质体介导基因转染的影响

目的]为非病毒载体阳离子类脂的研究提供参考。方法]以阳离子脂质体Lipofectamine 2000为载体,通过DNA延滞试验和质粒转染试验,研究Lipofectamine 2000对不同细胞(HeLa cell、B16 cell、MCF-7 cell和SW-480 cell)的转染效率,将其与其他转染试剂Sofast和DOTAP进行比较,并探讨细胞种类对Lipofectamine 2000转染效率的影响。结果]随着复合物中转染试剂比例的增加,DNA延滞作用明显增强。当Lipofectamine 2000与DNA的质量比增至51:时,仅有小部分DNA移出原点。Lipofectamine 2000对SW-480cell的转染效率明显高于其他3种细胞。Lipofectamine 2000对B16细胞株的转染效果要优于Sofast,而Sofast与DOTAP的转染效果相当。结论]Lipofectamine 2000对多数细胞具有较高的转染效率,并不适合所有类型的细胞。

Effects of Cell Types on Gene Transfection Mediated by Cationic Liposome

Objective] The research aimed to provide references for the research on non-viral vector cationic lipids.Method] With cationic liposome Lipofectamine 2000 as vector,the transfection efficiency of Lipofectamine 2000 to different cells(HeLa cell,B16 cell,MCF-7 cell and SW-480 cell) were studied through DNA delay test and plasmid transfection test and it was compared with that of other transfection reagents(Sofast and DOTAP).And the effects of cell types on the transfection efficiency of Lipofectamine 2000 were discussed.Result] With the increasing of the proportion of transfection reagent in the complex,DNA delay action was strengthened.When the mass ratio of Lipofectamine 2000 to DNA was increased to 5:1,only a few DNA moved out from the origin site.The transfection efficiency of Lipofectamine 2000 to SW-480 cell was obviously higher than other 3 kinds of cell types.The transfection effeiciency of Lipofectamine 2000 on B16 cell line was better than that of Sofast and that of Sofast and DOTAP was equivalent.Conclusion] Lipofectamine 2000 had high transfection efficiency to most cells,but it was not suitable for all types of cells.